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    dnali1-antibody-ab155490.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Motor Proteins Dynein
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Anti-DNALI1 antibody (ab155490)

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  • SDS
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Western blot - Anti-DNALI1 antibody (ab155490)
  • Immunocytochemistry/ Immunofluorescence - Anti-DNALI1 antibody (ab155490)

Key features and details

  • Rabbit polyclonal to DNALI1
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human DNALI1 protein (ab176067)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-DNALI1 antibody
    See all DNALI1 primary antibodies
  • Description

    Rabbit polyclonal to DNALI1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 14-258 of Human DNALI1 (Uniprot ID: O14645).

  • Positive control

    • A549 whole cell lysate; A549 cells.
  • General notes

    Keep as a concentrated solution.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Motor Proteins
    • Dynein

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • A549 whole cell lysate (ab7910)
  • Recombinant Protein

    • Recombinant Human DNALI1 protein (ab176067)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab155490 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
WB
Human
All applications
Mouse
Rat
Cow
Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 30 kDa.
ICC/IF
1/100 - 1/1000.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 30 kDa.
ICC/IF
1/100 - 1/1000.

Target

  • Function

    May play a dynamic role in flagellar motility.
  • Tissue specificity

    Expressed in many tissues. A smaller 0.9 kb and a larger 2.5 kb transcripts were detected at the highest level in the testis, at medium levels in the prostate, heart, liver, lung and pancreas, at low levels in the ovary, skeletal muscle and small intestine. Not detected in spleen, colon epithelium, thymus or peripheral blood leukocytes. The 0.9 kb transcript is expressed at a 20-fold higher level than the 2.5 kb transcript in the testis.
  • Sequence similarities

    Belongs to the inner dynein arm light chain family.
  • Target information above from: UniProt accession O14645 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 529053 Cow
    • Entrez Gene: 7802 Human
    • Entrez Gene: 75563 Mouse
    • Entrez Gene: 298524 Rat
    • Omim: 602135 Human
    • SwissProt: O14645 Human
    • SwissProt: Q8BVN8 Mouse
    • SwissProt: Q4FZV3 Rat
    • Unigene: 406050 Human
    • Unigene: 50304 Mouse
    • Unigene: 146067 Rat
    see all
  • Alternative names

    • axonemal antibody
    • Axonemal dynein light chain (hp28) antibody
    • Axonemal dynein light chain antibody
    • Axonemal dynein light intermediate polypeptide 1 antibody
    • dJ423B22.5 antibody
    • DNALI 1 antibody
    • DNALI1 antibody
    • Dynein axonemal light intermediate chain 1 antibody
    • Dynein axonemal light intermediate polypeptide 1 antibody
    • hp28 antibody
    • IDLC_HUMAN antibody
    • Inner dynein arm homolog of clamydomonas antibody
    • Inner dynein arm light chain antibody
    • Inner dynein arm light chain axonemal antibody
    • P28 antibody
    see all

Images

  • Western blot - Anti-DNALI1 antibody (ab155490)
    Western blot - Anti-DNALI1 antibody (ab155490)
    Anti-DNALI1 antibody (ab155490) at 1/1000 dilution + A549 whole cell lysate at 30 µg

    Predicted band size: 30 kDa



    12% SDS PAGE
  • Immunocytochemistry/ Immunofluorescence - Anti-DNALI1 antibody (ab155490)
    Immunocytochemistry/ Immunofluorescence - Anti-DNALI1 antibody (ab155490)
    Immunofluorescent analysis of methanol-fixed A549 cells labeling DNALI1 with ab155490 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.

Protocols

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab155490? Please let us know so that we can cite the reference in this datasheet.

    ab155490 has not yet been referenced specifically in any publications.

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