Anti-DNALI1 antibody (ab155490)
Key features and details
- Rabbit polyclonal to DNALI1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DNALI1 antibody
See all DNALI1 primary antibodies -
Description
Rabbit polyclonal to DNALI1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 14-258 of Human DNALI1 (Uniprot ID: O14645).
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Positive control
- A549 whole cell lysate; A549 cells.
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General notes
Keep as a concentrated solution.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155490 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| WB |
Human
|
| All applications |
Mouse
Rat
Cow
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 30 kDa.
|
|
| ICC/IF |
1/100 - 1/1000.
|
| Notes |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 30 kDa. |
|
ICC/IF
1/100 - 1/1000. |
Target
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Function
May play a dynamic role in flagellar motility. -
Tissue specificity
Expressed in many tissues. A smaller 0.9 kb and a larger 2.5 kb transcripts were detected at the highest level in the testis, at medium levels in the prostate, heart, liver, lung and pancreas, at low levels in the ovary, skeletal muscle and small intestine. Not detected in spleen, colon epithelium, thymus or peripheral blood leukocytes. The 0.9 kb transcript is expressed at a 20-fold higher level than the 2.5 kb transcript in the testis. -
Sequence similarities
Belongs to the inner dynein arm light chain family. - Information by UniProt
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Database links
- Entrez Gene: 529053 Cow
- Entrez Gene: 7802 Human
- Entrez Gene: 75563 Mouse
- Entrez Gene: 298524 Rat
- Omim: 602135 Human
- SwissProt: O14645 Human
- SwissProt: Q8BVN8 Mouse
- SwissProt: Q4FZV3 Rat
see all -
Alternative names
- axonemal antibody
- Axonemal dynein light chain (hp28) antibody
- Axonemal dynein light chain antibody
see all
Images
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Western blot - Anti-DNALI1 antibody (ab155490)Anti-DNALI1 antibody (ab155490) at 1/1000 dilution + A549 whole cell lysate at 30 µg
Predicted band size: 30 kDa
12% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-DNALI1 antibody (ab155490)Immunofluorescent analysis of methanol-fixed A549 cells labeling DNALI1 with ab155490 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.
Protocols
References (0)
ab155490 has not yet been referenced specifically in any publications.
