• Product name
  • Description
    Rabbit polyclonal to DNALI1
  • Host species
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (VTANKAHTGQ GSCWVATLAS AMIPPADSLL KYDTPVLVSR NTEKRSPKAR) of Human DNALI1 (NP_003453).

  • Positive control
    • Transfected 293T cell lysate.


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituents: PBS, 2% Sucrose
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab87075 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 30 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


  • Function
    May play a dynamic role in flagellar motility.
  • Tissue specificity
    Expressed in many tissues. A smaller 0.9 kb and a larger 2.5 kb transcripts were detected at the highest level in the testis, at medium levels in the prostate, heart, liver, lung and pancreas, at low levels in the ovary, skeletal muscle and small intestine. Not detected in spleen, colon epithelium, thymus or peripheral blood leukocytes. The 0.9 kb transcript is expressed at a 20-fold higher level than the 2.5 kb transcript in the testis.
  • Sequence similarities
    Belongs to the inner dynein arm light chain family.
  • Information by UniProt
  • Database links
  • Alternative names
    • axonemal antibody
    • Axonemal dynein light chain (hp28) antibody
    • Axonemal dynein light chain antibody
    • Axonemal dynein light intermediate polypeptide 1 antibody
    • dJ423B22.5 antibody
    • DNALI 1 antibody
    • DNALI1 antibody
    • Dynein axonemal light intermediate chain 1 antibody
    • Dynein axonemal light intermediate polypeptide 1 antibody
    • hp28 antibody
    • IDLC_HUMAN antibody
    • Inner dynein arm homolog of clamydomonas antibody
    • Inner dynein arm light chain antibody
    • Inner dynein arm light chain axonemal antibody
    • P28 antibody
    see all


  • Species+tissue/cell type:Human nasal epithelial cells. Primary antibody dilution: 1:1000. Secondary antibody: Goat anti-rabbit Alexa Fluor 546. Secondary antibody dilution:1:1000

  • Anti-DNALI1 antibody (ab87075) at 1 µg/ml (in 5% skim milk / PBS buffer) + Transfected 293T cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 30 kDa
    Observed band size: 32 kDa
    why is the actual band size different from the predicted?

    Gel concentration: 12%


This product has been referenced in:
  • Miao C  et al. Mutations in the Motile Cilia Gene DNAAF1 Are Associated with Neural Tube Defects in Humans. G3 (Bethesda) 6:3307-3316 (2016). ICC/IF . Read more (PubMed: 27543293) »
  • Jeanson L  et al. RSPH3 Mutations Cause Primary Ciliary Dyskinesia with Central-Complex Defects and a Near Absence of Radial Spokes. Am J Hum Genet 97:153-62 (2015). Read more (PubMed: 26073779) »
See all 2 Publications for this product

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