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Rabbit polyclonal to DNase gamma
Recombinant fragment, corresponding to a region within amino acids 115-305 of Human DNase gamma (UniProt; Q13609).
- A549, HeLa, HepG2 and HCT116 whole cell lysates; HeLa cells.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/500 - 1/3000. Predicted molecular weight: 36 kDa.
||1/100 - 1/1000.
Has DNA hydrolytic activity. Does not bind to actin. Cleaves chromatin DNA to nucleosomal units.
Liver and spleen.
Belongs to the DNase I family.
Nucleus. May first pass through the ER membrane before being imported in the nucleus.
Information by UniProt
- Deoxyribonuclease gamma antibody
- Deoxyribonuclease I like 3 antibody
- Deoxyribonuclease I like III antibody
Western blot - Anti-DNase gamma antibody (ab152118)
Anti-DNase gamma antibody (ab152118) at 1/1000 dilution + HCT116 whole cell lysate at 30 µg
Predicted band size: 36 kDa
10% SDS PAGE
Immunocytochemistry/ Immunofluorescence - Anti-DNase gamma antibody (ab152118)
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells, labeling DNase gamma with ab152118 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
has not yet been referenced specifically in any publications.
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