Anti-DNase gamma antibody (ab152118)
Key features and details
- Rabbit polyclonal to DNase gamma
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DNase gamma antibody
See all DNase gamma primary antibodies -
Description
Rabbit polyclonal to DNase gamma -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 115-305 of Human DNase gamma (UniProt; Q13609).
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Positive control
- A549, HeLa, HepG2 and HCT116 whole cell lysates; HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab152118 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 36 kDa.
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| ICC/IF |
1/100 - 1/1000.
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| Notes |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 36 kDa. |
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ICC/IF
1/100 - 1/1000. |
Target
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Function
Has DNA hydrolytic activity. Does not bind to actin. Cleaves chromatin DNA to nucleosomal units. -
Tissue specificity
Liver and spleen. -
Sequence similarities
Belongs to the DNase I family. -
Cellular localization
Nucleus. May first pass through the ER membrane before being imported in the nucleus. - Information by UniProt
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Database links
- Entrez Gene: 1776 Human
- Omim: 602244 Human
- SwissProt: Q13609 Human
- Unigene: 476453 Human
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Alternative names
- Deoxyribonuclease gamma antibody
- Deoxyribonuclease I like 3 antibody
- Deoxyribonuclease I like III antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-DNase gamma antibody (ab152118)
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Immunocytochemistry/ Immunofluorescence - Anti-DNase gamma antibody (ab152118)
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Immunocytochemistry/ Immunofluorescence - Anti-DNase gamma antibody (ab152118)
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Immunocytochemistry/ Immunofluorescence - Anti-DNase gamma antibody (ab152118)
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Immunocytochemistry/ Immunofluorescence - Anti-DNase gamma antibody (ab152118)
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Western blot - Anti-DNase gamma antibody (ab152118)Anti-DNase gamma antibody (ab152118) at 1/1000 dilution + HCT116 whole cell lysate at 30 µg
Predicted band size: 36 kDa
10% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-DNase gamma antibody (ab152118)Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells, labeling DNase gamma with ab152118 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab152118 has been referenced in 1 publication.
- Lazzaretto B & Fadeel B Intra- and Extracellular Degradation of Neutrophil Extracellular Traps by Macrophages and Dendritic Cells. J Immunol 203:2276-2290 (2019). PubMed: 31519860