Anti-DNase I antibody [6D5] (ab205567)
![Western blot - Anti-DNase I antibody [6D5] (ab205567)](https://www.abcam.com/ps/products/205/ab205567/Images/ab205567-248614-anti-dnase-i-antibody-6d5-western-blot.jpg "Western blot - Anti-DNase I antibody [6D5] (ab205567)")
Key features and details
- Mouse monoclonal [6D5] to DNase I
- Suitable for: WB
- Reacts with: Chinese hamster
- Isotype: IgG1
Overview
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Product name
Anti-DNase I antibody [6D5]
See all DNase I primary antibodies -
Description
Mouse monoclonal [6D5] to DNase I -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Chinese hamster
Predicted to work with: Human
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Immunogen
Recombinant full length protein corresponding to Cow DNase I. Recombinant full length bovine DNaseI produced using CHO-based proprietary suspension cell line.
Database link: P00639 -
Positive control
- Recombinant bovine DNaseI expressed into the supernatant of CHO cell culture medium.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
ab205567 is purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
6D5 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab205567 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 31 kDa.
WB: 0.1 to 0.2 μg/ml (bovine DNaseI) or 1 μg/ml (Human DNaseI) |
| Notes |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 31 kDa. WB: 0.1 to 0.2 μg/ml (bovine DNaseI) or 1 μg/ml (Human DNaseI) |
Target
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Function
Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization. -
Tissue specificity
Principally in tissues of the digestive system. Highest levels found in urine, but also relatively abundant in semen and saliva. -
Sequence similarities
Belongs to the DNase I family. -
Cellular localization
Secreted. Nucleus envelope. Secretory protein, stored in zymogen granules and found in the nuclear envelope. - Information by UniProt
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Database links
- Entrez Gene: 1773 Human
- Omim: 125505 Human
- SwissProt: P24855 Human
- Unigene: 629638 Human
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Alternative names
- Deoxyribonuclease 1 antibody
- Deoxyribonuclease I antibody
- Deoxyribonuclease-1 antibody
see all
Images
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Western blot - Anti-DNase I antibody [6D5] (ab205567)Anti-DNase I antibody [6D5] (ab205567) at 0.2 µg/ml + Recombinant bovine DNaseI expressed into the supernatant of CHO cell culture medium. at 10 µl
Predicted band size: 31 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab205567 has not yet been referenced specifically in any publications.