Anti-DNase I antibody [6D5] (ab205567)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-DNase I antibody [6D5]
See all DNase I primary antibodies -
DescriptionMouse monoclonal [6D5] to DNase I
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Host speciesMouse
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Cow
Predicted to work with: Human
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Immunogen
Recombinant full length protein corresponding to Cow DNase I. Recombinant full length bovine DNaseI produced using CHO-based proprietary suspension cell line.
Database link: P00639 -
Positive control
- Recombinant bovine DNaseI expressed into the supernatant of CHO cell culture medium.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.40
Constituent: 99% PBS -
Concentration information loading... -
PurityProtein G purified
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Purification notesab205567 is purified from cell culture supernatant.
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ClonalityMonoclonal
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Clone number6D5
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IsotypeIgG1
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab205567 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Predicted molecular weight: 31 kDa. WB: 0.1 to 0.2 μg/ml (bovine DNaseI) or 1 μg/ml (Human DNaseI) |
Target
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FunctionAmong other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization.
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Tissue specificityPrincipally in tissues of the digestive system. Highest levels found in urine, but also relatively abundant in semen and saliva.
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Sequence similaritiesBelongs to the DNase I family.
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Cellular localizationSecreted. Nucleus envelope. Secretory protein, stored in zymogen granules and found in the nuclear envelope.
- Information by UniProt
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Database links
- Entrez Gene: 282217 Cow
- Entrez Gene: 1773 Human
- Omim: 125505 Human
- SwissProt: P00639 Cow
- SwissProt: P24855 Human
- Unigene: 629638 Human
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Alternative names
- Deoxyribonuclease 1 antibody
- Deoxyribonuclease I antibody
- Deoxyribonuclease-1 antibody
see all
Images
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![Western blot - Anti-DNase I antibody [6D5] (ab205567)](https://www.abcam.com/ps/products/205/ab205567/Images/ab205567-248614-205567.JPG)
Western blot - Anti-DNase I antibody [6D5] (ab205567)Anti-DNase I antibody [6D5] (ab205567) at 0.2 µg/ml + Recombinant bovine DNaseI expressed into the supernatant of CHO cell culture medium. at 10 µl
Predicted band size: 31 kDa
Protocols
Datasheets and documents
References
ab205567 has not yet been referenced specifically in any publications.
