Anti-DNase I antibody (ab113241)
- Datasheet
- References (3)
- Protocols
Overview
-
Product name
Anti-DNase I antibody
See all DNase I primary antibodies -
Description
Rabbit polyclonal to DNase I -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ELISAmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant Human DNase I protein.
-
Positive control
- Human Brain cerebellum and Tonsil tissues; MOLT4 cell lysate.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.025% CMIT/MIT 3:1
Constituents: 20% Glycerol, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab113241 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 31 kDa. | |
| IHC-P | Use a concentration of 10 µg/ml. | |
| ELISA | Use at an assay dependent concentration. |
Target
-
Function
Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization. -
Tissue specificity
Principally in tissues of the digestive system. Highest levels found in urine, but also relatively abundant in semen and saliva. -
Sequence similarities
Belongs to the DNase I family. -
Cellular localization
Secreted. Nucleus envelope. Secretory protein, stored in zymogen granules and found in the nuclear envelope. - Information by UniProt
-
Database links
- Entrez Gene: 1773 Human
- Omim: 125505 Human
- SwissProt: P24855 Human
- Unigene: 629638 Human
-
Alternative names
- Deoxyribonuclease 1 antibody
- Deoxyribonuclease I antibody
- Deoxyribonuclease-1 antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNase I antibody (ab113241)ab113241, at 10 µg/ml, staining DNase I in Formalin-fixed, Paraffin-embedded Human Brain cerebellum tissue by Immunohistochemistry. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNase I antibody (ab113241)ab113241, at 10 µg/ml, staining DNase I in Formalin-fixed, Paraffin-embedded Human Tonsil tissue by Immunohistochemistry. -
Western blot - Anti-DNase I antibody (ab113241)Anti-DNase I antibody (ab113241) at 1/1000 dilution + MOLT4 cell lysate at 30 µg
Predicted band size: 31 kDa
10% SDS PAGE
References
This product has been referenced in:
- Thiyagarajan D et al. IL-1ß Promotes a New Function of DNase I as a Transcription Factor for the Fas Receptor Gene. Front Cell Dev Biol 6:7 (2018). Read more (PubMed: 29468159) »
- Pedersen HL et al. Lupus nephritis: low urinary DNase I levels reflect loss of renal DNase I and may be utilized as a biomarker of disease progression. J Pathol Clin Res 4:193-203 (2018). Read more (PubMed: 29624903) »
