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Cell Biology Apoptosis Nucleus DNAses
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Anti-DNase I antibody (ab113241)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (3)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNase I antibody (ab113241)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNase I antibody (ab113241)
  • Western blot - Anti-DNase I antibody (ab113241)

Key features and details

  • Rabbit polyclonal to DNase I
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-DNase I antibody
    See all DNase I primary antibodies
  • Description

    Rabbit polyclonal to DNase I
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant Human DNase I protein.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 20% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Nucleus
    • DNAses
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Apoptosis
    • Nuclear
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Nucleus
    • DNAses
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Associated products

  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • MOLT-4 whole cell lysate (ab7912)
  • Recombinant Protein

    • Recombinant human DNase I protein (Active) (ab73430)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab113241 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 31 kDa.
IHC-P
Use a concentration of 10 µg/ml.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 31 kDa.
IHC-P
Use a concentration of 10 µg/ml.

Target

  • Function

    Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization.
  • Tissue specificity

    Principally in tissues of the digestive system. Highest levels found in urine, but also relatively abundant in semen and saliva.
  • Sequence similarities

    Belongs to the DNase I family.
  • Cellular localization

    Secreted. Nucleus envelope. Secretory protein, stored in zymogen granules and found in the nuclear envelope.
  • Target information above from: UniProt accession P24855 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1773 Human
    • Omim: 125505 Human
    • SwissProt: P24855 Human
    • Unigene: 629638 Human
    • Alternative names

      • Deoxyribonuclease 1 antibody
      • Deoxyribonuclease I antibody
      • Deoxyribonuclease-1 antibody
      • Deoxyribonuclease1 antibody
      • DeoxyribonucleaseI antibody
      • DNAS1_HUMAN antibody
      • DNASE 1 antibody
      • DNase I antibody
      • DNase I lysosomal antibody
      • DNASE1 antibody
      • DNaseI antibody
      • DNL 1 antibody
      • DNL1 antibody
      • Dornase alfa antibody
      • DRNI antibody
      • FLJ38093 antibody
      • Human urine deoxyribonuclease I antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNase I antibody (ab113241)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNase I antibody (ab113241)
      ab113241, at 10 µg/ml, staining DNase I in Formalin-fixed, Paraffin-embedded Human Brain cerebellum tissue by Immunohistochemistry.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNase I antibody (ab113241)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNase I antibody (ab113241)
      ab113241, at 10 µg/ml, staining DNase I in Formalin-fixed, Paraffin-embedded Human Tonsil tissue by Immunohistochemistry.
    • Western blot - Anti-DNase I antibody (ab113241)
      Western blot - Anti-DNase I antibody (ab113241)
      Anti-DNase I antibody (ab113241) at 1/1000 dilution + MOLT4 cell lysate at 30 µg

      Predicted band size: 31 kDa



      10% SDS PAGE

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (3)

    Publishing research using ab113241? Please let us know so that we can cite the reference in this datasheet.

    ab113241 has been referenced in 3 publications.

    • Thiyagarajan D  et al. IL-1ß Promotes a New Function of DNase I as a Transcription Factor for the Fas Receptor Gene. Front Cell Dev Biol 6:7 (2018). PubMed: 29468159
    • Pedersen HL  et al. Lupus nephritis: low urinary DNase I levels reflect loss of renal DNase I and may be utilized as a biomarker of disease progression. J Pathol Clin Res 4:193-203 (2018). PubMed: 29624903
    • Fismen S  et al. Impact of the Tumor Necrosis Factor Receptor-Associated Protein 1 (Trap1) on Renal DNaseI Shutdown and on Progression of Murine and Human Lupus Nephritis. Am J Pathol 182:688-700 (2013). PubMed: 23273922

    Customer reviews and Q&As

    Show All Reviews Q&A
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    ELISA abreview for Anti-DNase I antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    ELISA
    Sample
    Human Recombinant protein (rhDnase)
    Specification
    rhDnase
    Type
    Indirect
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 37°C
    Read More

    Maria Machado

    Verified customer

    Submitted Oct 29 2014

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