Anti-DNase I antibody (ab113241)
Key features and details
- Rabbit polyclonal to DNase I
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DNase I antibody
See all DNase I primary antibodies -
Description
Rabbit polyclonal to DNase I -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Recombinant Human DNase I protein.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 20% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab113241 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-P |
Human
|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 31 kDa.
|
|
| IHC-P |
Use a concentration of 10 µg/ml.
|
| Notes |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 31 kDa. |
|
IHC-P
Use a concentration of 10 µg/ml. |
Target
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Function
Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization. -
Tissue specificity
Principally in tissues of the digestive system. Highest levels found in urine, but also relatively abundant in semen and saliva. -
Sequence similarities
Belongs to the DNase I family. -
Cellular localization
Secreted. Nucleus envelope. Secretory protein, stored in zymogen granules and found in the nuclear envelope. - Information by UniProt
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Database links
- Entrez Gene: 1773 Human
- Omim: 125505 Human
- SwissProt: P24855 Human
- Unigene: 629638 Human
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Alternative names
- Deoxyribonuclease 1 antibody
- Deoxyribonuclease I antibody
- Deoxyribonuclease-1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNase I antibody (ab113241)ab113241, at 10 µg/ml, staining DNase I in Formalin-fixed, Paraffin-embedded Human Brain cerebellum tissue by Immunohistochemistry.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNase I antibody (ab113241)ab113241, at 10 µg/ml, staining DNase I in Formalin-fixed, Paraffin-embedded Human Tonsil tissue by Immunohistochemistry. -
Western blot - Anti-DNase I antibody (ab113241)Anti-DNase I antibody (ab113241) at 1/1000 dilution + MOLT4 cell lysate at 30 µg
Predicted band size: 31 kDa
10% SDS PAGE
Protocols
References (3)
ab113241 has been referenced in 3 publications.
- Thiyagarajan D et al. IL-1ß Promotes a New Function of DNase I as a Transcription Factor for the Fas Receptor Gene. Front Cell Dev Biol 6:7 (2018). PubMed: 29468159
- Pedersen HL et al. Lupus nephritis: low urinary DNase I levels reflect loss of renal DNase I and may be utilized as a biomarker of disease progression. J Pathol Clin Res 4:193-203 (2018). PubMed: 29624903
- Fismen S et al. Impact of the Tumor Necrosis Factor Receptor-Associated Protein 1 (Trap1) on Renal DNaseI Shutdown and on Progression of Murine and Human Lupus Nephritis. Am J Pathol 182:688-700 (2013). PubMed: 23273922
