Overview

  • Product name
    Anti-DNase I antibody
    See all DNase I primary antibodies
  • Description
    Rabbit polyclonal to DNase I
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant Human DNase I protein.

  • Positive control
    • Human Brain cerebellum and Tonsil tissues; MOLT4 cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab113241 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 31 kDa.
IHC-P Use a concentration of 10 µg/ml.
ELISA Use at an assay dependent concentration.

Target

  • Function
    Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization.
  • Tissue specificity
    Principally in tissues of the digestive system. Highest levels found in urine, but also relatively abundant in semen and saliva.
  • Sequence similarities
    Belongs to the DNase I family.
  • Cellular localization
    Secreted. Nucleus envelope. Secretory protein, stored in zymogen granules and found in the nuclear envelope.
  • Information by UniProt
  • Database links
    • Alternative names
      • Deoxyribonuclease 1 antibody
      • Deoxyribonuclease I antibody
      • Deoxyribonuclease-1 antibody
      • Deoxyribonuclease1 antibody
      • DeoxyribonucleaseI antibody
      • DNAS1_HUMAN antibody
      • DNASE 1 antibody
      • DNase I antibody
      • DNase I lysosomal antibody
      • DNASE1 antibody
      • DNaseI antibody
      • DNL 1 antibody
      • DNL1 antibody
      • Dornase alfa antibody
      • DRNI antibody
      • FLJ38093 antibody
      • Human urine deoxyribonuclease I antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNase I antibody (ab113241)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNase I antibody (ab113241)
      ab113241, at 10 µg/ml, staining DNase I in Formalin-fixed, Paraffin-embedded Human Brain cerebellum tissue by Immunohistochemistry.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNase I antibody (ab113241)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNase I antibody (ab113241)
      ab113241, at 10 µg/ml, staining DNase I in Formalin-fixed, Paraffin-embedded Human Tonsil tissue by Immunohistochemistry.
    • Western blot - Anti-DNase I antibody (ab113241)
      Western blot - Anti-DNase I antibody (ab113241)
      Anti-DNase I antibody (ab113241) at 1/1000 dilution + MOLT4 cell lysate at 30 µg

      Predicted band size: 31 kDa



      10% SDS PAGE

    References

    This product has been referenced in:
    • Thiyagarajan D  et al. IL-1ß Promotes a New Function of DNase I as a Transcription Factor for the Fas Receptor Gene. Front Cell Dev Biol 6:7 (2018). Read more (PubMed: 29468159) »
    • Fismen S  et al. Impact of the Tumor Necrosis Factor Receptor-Associated Protein 1 (Trap1) on Renal DNaseI Shutdown and on Progression of Murine and Human Lupus Nephritis. Am J Pathol 182:688-700 (2013). Read more (PubMed: 23273922) »

    See all 2 Publications for this product

    Publishing research using ab113241? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1 Review or Q&A

    10
    Votes: Highest First

    ELISA abreview for Anti-DNase I antibody

     Excellent
    Abreviews
    Application
    ELISA
    Sample
    Human Recombinant protein (rhDnase)
    Specification
    rhDnase
    Type
    Indirect
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 37°C
    Read More
    abreview image
    Username

    Maria Machado

    Verified customer

    Submitted Oct 29 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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