Anti-DNase I antibody (ab113241)
Key features and details
- Rabbit polyclonal to DNase I
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DNase I antibody
See all DNase I primary antibodies -
Description
Rabbit polyclonal to DNase I -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant Human DNase I protein.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 20% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab113241 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 31 kDa.
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| IHC-P |
Use a concentration of 10 µg/ml.
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| Notes |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 31 kDa. |
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IHC-P
Use a concentration of 10 µg/ml. |
Target
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Function
Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization. -
Tissue specificity
Principally in tissues of the digestive system. Highest levels found in urine, but also relatively abundant in semen and saliva. -
Sequence similarities
Belongs to the DNase I family. -
Cellular localization
Secreted. Nucleus envelope. Secretory protein, stored in zymogen granules and found in the nuclear envelope. - Information by UniProt
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Database links
- Entrez Gene: 1773 Human
- Omim: 125505 Human
- SwissProt: P24855 Human
- Unigene: 629638 Human
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Alternative names
- Deoxyribonuclease 1 antibody
- Deoxyribonuclease I antibody
- Deoxyribonuclease-1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNase I antibody (ab113241)ab113241, at 10 µg/ml, staining DNase I in Formalin-fixed, Paraffin-embedded Human Brain cerebellum tissue by Immunohistochemistry.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNase I antibody (ab113241)ab113241, at 10 µg/ml, staining DNase I in Formalin-fixed, Paraffin-embedded Human Tonsil tissue by Immunohistochemistry. -
Western blot - Anti-DNase I antibody (ab113241)Anti-DNase I antibody (ab113241) at 1/1000 dilution + MOLT4 cell lysate at 30 µg
Predicted band size: 31 kDa
10% SDS PAGE
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab113241 has been referenced in 3 publications.
- Thiyagarajan D et al. IL-1ß Promotes a New Function of DNase I as a Transcription Factor for the Fas Receptor Gene. Front Cell Dev Biol 6:7 (2018). PubMed: 29468159
- Pedersen HL et al. Lupus nephritis: low urinary DNase I levels reflect loss of renal DNase I and may be utilized as a biomarker of disease progression. J Pathol Clin Res 4:193-203 (2018). PubMed: 29624903
- Fismen S et al. Impact of the Tumor Necrosis Factor Receptor-Associated Protein 1 (Trap1) on Renal DNaseI Shutdown and on Progression of Murine and Human Lupus Nephritis. Am J Pathol 182:688-700 (2013). PubMed: 23273922