Application
ELISA
Sample
Human Recombinant protein (rhDnase)
Specification
rhDnase
Type
Indirect
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 37°C
Other product details
Incubation time
1 hour(s) and 30 minute(s) · Temperature: 20°C · Diluent: PBST 0,1%
Dilution
1/2000
Additional data
Additional Notes
Coating with streptavidin 5´g/ml in NaHCO3 ph 9,4 over night at 4¯C. RhDnase has to be biotinylated first.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Maria Machado
Verified customer
Submitted Oct 29 2014