Overview

  • Product name
  • Description
    Rabbit polyclonal to DNase I
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Cow
  • Immunogen

    Recombinant full length protein corresponding to Cow DNase I. Recombinant full length bovine DNaseI produced using CHO-based proprietary suspension cell line.
    Database link: P00639

  • Positive control
    • Recombinant bovine DNaseI expressed into the supernatant of CHO cell culture medium.
  • General notes

    As product is (NH4)2SO4 precipitate, mix well by pipetting or vortexing prior use. 

Properties

Applications

Our Abpromise guarantee covers the use of ab205566 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.16 µg/ml. Predicted molecular weight: 31 kDa.

Target

  • Function
    Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization.
  • Tissue specificity
    Principally in tissues of the digestive system. Highest levels found in urine, but also relatively abundant in semen and saliva.
  • Sequence similarities
    Belongs to the DNase I family.
  • Cellular localization
    Secreted. Nucleus envelope. Secretory protein, stored in zymogen granules and found in the nuclear envelope.
  • Information by UniProt
  • Database links
  • Alternative names
    • Deoxyribonuclease 1 antibody
    • Deoxyribonuclease I antibody
    • Deoxyribonuclease-1 antibody
    • Deoxyribonuclease1 antibody
    • DeoxyribonucleaseI antibody
    • DNAS1_HUMAN antibody
    • DNASE 1 antibody
    • DNase I antibody
    • DNase I lysosomal antibody
    • DNASE1 antibody
    • DNaseI antibody
    • DNL 1 antibody
    • DNL1 antibody
    • Dornase alfa antibody
    • DRNI antibody
    • FLJ38093 antibody
    • Human urine deoxyribonuclease I antibody
    see all

Images

  • Anti-DNase I antibody (ab205566) at 0.1 µg/ml + Recombinant bovine DNaseI expressed into the supernatant of CHO cell culture medium.

    Predicted band size: 31 kDa

References

ab205566 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (MEFs)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
20 µg
Specification
MEFs
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C

Abcam user community

Verified customer

Submitted Jul 27 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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