Key features and details
- Rabbit polyclonal to DNase I
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-DNase I antibody
See all DNase I primary antibodies
DescriptionRabbit polyclonal to DNase I
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
- WB: Mouse brain tissue lysate. IHC-P: Human ovarian cancer tissue.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab224617 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Detects a band of approximately 32 kDa (predicted molecular weight: 31 kDa).|
|IHC-P||1/20 - 1/200.|
FunctionAmong other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization.
Tissue specificityPrincipally in tissues of the digestive system. Highest levels found in urine, but also relatively abundant in semen and saliva.
Sequence similaritiesBelongs to the DNase I family.
Cellular localizationSecreted. Nucleus envelope. Secretory protein, stored in zymogen granules and found in the nuclear envelope.
- Information by UniProt
- Deoxyribonuclease 1 antibody
- Deoxyribonuclease I antibody
- Deoxyribonuclease-1 antibody
Paraffin-embedded human ovarian cancer tissue stained for DNase I using ab224617 at 1/100 dilution in immunohistochemical analysis.
Anti-DNase I antibody (ab224617) at 1/500 dilution + Mouse brain tissue lysate
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 31 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab224617 has not yet been referenced specifically in any publications.