Key features and details
- Rabbit polyclonal to DNase I
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DNase I antibody
See all DNase I primary antibodies
DescriptionRabbit polyclonal to DNase I
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Recombinant protein fragment containing a sequence corresponding to a region within amino acids 1 and 252 of Human DNase I (NP_005214).
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab97393 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 31 kDa.|
FunctionAmong other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization.
Tissue specificityPrincipally in tissues of the digestive system. Highest levels found in urine, but also relatively abundant in semen and saliva.
Sequence similaritiesBelongs to the DNase I family.
Cellular localizationSecreted. Nucleus envelope. Secretory protein, stored in zymogen granules and found in the nuclear envelope.
- Information by UniProt
- Deoxyribonuclease 1 antibody
- Deoxyribonuclease I antibody
- Deoxyribonuclease-1 antibody
Anti-DNase I antibody (ab97393) at 1/1000 dilution + Molt-4 whole cell lysate at 30 µg
Predicted band size: 31 kDa
10% SDS PAGE
Anti-DNase I antibody (ab97393) at 1/1000 dilution +
Recombinant human DNase I protein (Active) (ab73430) at 0.001 µg
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 31 kDa
Exposure time: 4 minutes
ab97393 has not yet been referenced specifically in any publications.