The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 40 kDa.
Use a concentration of 10 µg/ml.
Hydrolyzes DNA under acidic conditions with a preference for double-stranded DNA. Plays a major role in the degradation of nuclear DNA in cellular apoptosis during development. Necessary for proper fetal development and for definitive erythropoiesis in fetal liver, where it degrades nuclear DNA expelled from erythroid precursor cells.
Belongs to the DNase II family.
Glycosylated. Mutations that eliminate N-glycosylation sites reduce activity, but enzymatic deglycosylation has no effect.
All lanes : Anti-DNase II antibody (ab168782) at 1 µg/ml
Lane 1 : DNase II transfected 293T cell lysate Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Predicted band size: 40 kDa
Immunocytochemistry/ Immunofluorescence - Anti-DNase II antibody (ab168782)
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling DNase II using ab168782 at 10 µg/ml. Cells should be permeabilized by incubating for 15 minutes on ice with 2 mL of 0.1% Triton X-100 in PBS prior to staining.