Anti-DNASE2 antibody (ab168782)

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Overview

  • Product name
    Anti-DNASE2 antibody
    See all DNASE2 primary antibodies
  • Description
    Mouse polyclonal to DNASE2
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human DNASE2 aa 1-360.
    Sequence:

    MIPLLLAALLCVPAGALTCYGDSGQPVDWFVVYKLPALRGSGEAAQRGLQ YKYLDESSGGWRDGRALINSPEGAVGRSLQPLYRSNTSQLAFLLYNDQPP QPSKAQDSSMRGHTKGVLLLDHDGGFWLVHSVPNFPPPASSAAYSWPHSA CTYGQTLLCVSFPFAQFSKMGKQLTYTYPWVYNYQLEGIFAQEFPDLENV VKGHHVSQEPWNSSITLTSQAGAVFQSFAKFSKFGDDLYSGWLAAALGTN LQVQFWHKTVGILPSNCSDIWQVLNVNQIAFPGPAGPSFNSTEDHSKWCV SPKGPWTCVGDMNRNQGEEQRGGGTLCAQLPALWKAFQPLVKNYQPCNGM ARKPSRAYKI


    Database link: NP_001366.1

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Lysate from DNASE2 transfected 293T cells; HeLa cells
  • General notes

     This product was previously labelled as DNase II

     

Properties

Applications

Our Abpromise guarantee covers the use of ab168782 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 40 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    Hydrolyzes DNA under acidic conditions with a preference for double-stranded DNA. Plays a major role in the degradation of nuclear DNA in cellular apoptosis during development. Necessary for proper fetal development and for definitive erythropoiesis in fetal liver, where it degrades nuclear DNA expelled from erythroid precursor cells.
  • Sequence similarities
    Belongs to the DNase II family.
  • Post-translational
    modifications
    Glycosylated. Mutations that eliminate N-glycosylation sites reduce activity, but enzymatic deglycosylation has no effect.
  • Cellular localization
    Lysosome.
  • Information by UniProt
  • Database links
    • Alternative names
      • Acid DNase antibody
      • Deoxyribonuclease 2 alpha antibody
      • Deoxyribonuclease 2 antibody
      • Deoxyribonuclease II alpha antibody
      • Deoxyribonuclease II antibody
      • Deoxyribonuclease II lysosomal antibody
      • Deoxyribonuclease-2-alpha antibody
      • Deoxyribonuclease2 antibody
      • DeoxyribonucleaseII antibody
      • DNASE 2 antibody
      • DNASE 2A antibody
      • DNase II alpha antibody
      • DNase II lysosomal antibody
      • DNASE2 antibody
      • DNASE2A antibody
      • DNaseII antibody
      • DNL 2 antibody
      • DNL antibody
      • DNL2 antibody
      • DNS2A_HUMAN antibody
      • Lysosomal DNase II antibody
      • R31240 2 antibody
      • R31240_2 antibody
      see all

    Images

    • Western blot - Anti-DNASE2 antibody (ab168782)
      Western blot - Anti-DNASE2 antibody (ab168782)
      All lanes : Anti-DNASE2 antibody (ab168782) at 1 µg/ml

      Lane 1 : DNASE2 transfected 293T cell lysate
      Lane 2 : Non transfected 293T cell lysate

      Lysates/proteins at 15 µl per lane.

      Predicted band size: 40 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-DNASE2 antibody (ab168782)
      Immunocytochemistry/ Immunofluorescence - Anti-DNASE2 antibody (ab168782)

      Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling DNASE2 using ab168782 at 10 µg/ml. Cells should be permeabilized by incubating for 15 minutes on ice with 2 mL of 0.1% Triton X-100 in PBS prior to staining.

    References

    ab168782 has not yet been referenced specifically in any publications.

    Publishing research using ab168782? Please let us know so that we can cite the reference in this datasheet.

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