Anti-DNASE2 antibody (ab168782)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-DNASE2 antibody
See all DNASE2 primary antibodies -
Description
Mouse polyclonal to DNASE2 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human DNASE2 aa 1-360.
Sequence:MIPLLLAALLCVPAGALTCYGDSGQPVDWFVVYKLPALRGSGEAAQRGLQ YKYLDESSGGWRDGRALINSPEGAVGRSLQPLYRSNTSQLAFLLYNDQPP QPSKAQDSSMRGHTKGVLLLDHDGGFWLVHSVPNFPPPASSAAYSWPHSA CTYGQTLLCVSFPFAQFSKMGKQLTYTYPWVYNYQLEGIFAQEFPDLENV VKGHHVSQEPWNSSITLTSQAGAVFQSFAKFSKFGDDLYSGWLAAALGTN LQVQFWHKTVGILPSNCSDIWQVLNVNQIAFPGPAGPSFNSTEDHSKWCV SPKGPWTCVGDMNRNQGEEQRGGGTLCAQLPALWKAFQPLVKNYQPCNGM ARKPSRAYKI
Database link: NP_001366.1 -
Positive control
- Lysate from DNASE2 transfected 293T cells; HeLa cells
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General notes
This product was previously labelled as DNase II
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.20
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab168782 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 40 kDa. | |
| ICC/IF | Use a concentration of 10 µg/ml. |
Target
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Function
Hydrolyzes DNA under acidic conditions with a preference for double-stranded DNA. Plays a major role in the degradation of nuclear DNA in cellular apoptosis during development. Necessary for proper fetal development and for definitive erythropoiesis in fetal liver, where it degrades nuclear DNA expelled from erythroid precursor cells. -
Sequence similarities
Belongs to the DNase II family. -
Post-translational
modificationsGlycosylated. Mutations that eliminate N-glycosylation sites reduce activity, but enzymatic deglycosylation has no effect. -
Cellular localization
Lysosome. - Information by UniProt
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Database links
- Entrez Gene: 1777 Human
- Omim: 126350 Human
- SwissProt: O00115 Human
- Unigene: 118243 Human
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Alternative names
- Acid DNase antibody
- Deoxyribonuclease 2 alpha antibody
- Deoxyribonuclease 2 antibody
see all
Images
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Western blot - Anti-DNASE2 antibody (ab168782)All lanes : Anti-DNASE2 antibody (ab168782) at 1 µg/ml
Lane 1 : DNASE2 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Predicted band size: 40 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-DNASE2 antibody (ab168782)Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling DNASE2 using ab168782 at 10 µg/ml. Cells should be permeabilized by incubating for 15 minutes on ice with 2 mL of 0.1% Triton X-100 in PBS prior to staining.
Datasheets and documents
References
ab168782 has not yet been referenced specifically in any publications.
