Overview

  • Product name
    Anti-DNHD1 antibody
  • Description
    Rabbit polyclonal to DNHD1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: KHSQATQPML ILLPPPGHPS ATLHPLTVIQ KLAAKYQQGQ KQLQVIALGS EAWDPVSVVV STLSQAMYEG HWLVLDNCHL MPHW, corresponding to internal sequence amino acids 4080-4163 of Human DNHD1.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Human lung tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

  • Sequence similarities
    Belongs to the dynein heavy chain family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • Database links
    • Alternative names
      • C11orf47 antibody
      • CCDC35 antibody
      • coiled-coil domain containing 35 antibody
      • DHCD1 antibody
      • DNHD1 antibody
      • DNHD1 variant protein antibody
      • DNHD1_HUMAN antibody
      • DNHD1L antibody
      • Dynein heavy chain domain 1-like protein antibody
      • Dynein heavy chain domain-containing protein 1 antibody
      • FLJ32752 antibody
      • FLJ35709 antibody
      • FLJ46184 antibody
      • Protein CCDC35 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-DNHD1 antibody (ab122574)
      Immunocytochemistry/ Immunofluorescence - Anti-DNHD1 antibody (ab122574)
      Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleoli and nucleus but not nucleoli. Recommended concentration of ab122574 1-4 µg/ml. Cells treated with PFA/Triton X-100.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNHD1 antibody (ab122574)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNHD1 antibody (ab122574)
      ab122574, at 1/50 dilution, staining DNHD1 in paraffin-embedded Human lung tissue by Immunohistochemistry.

    References

    ab122574 has not yet been referenced specifically in any publications.

    Publishing research using ab122574? Please let us know so that we can cite the reference in this datasheet.

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