Key features and details
- Rabbit polyclonal to DNMBP
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DNMBP antibody
See all DNMBP primary antibodies
DescriptionRabbit polyclonal to DNMBP
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Cynomolgus monkey, Rhesus monkey, Orangutan
- HeLa whole cell lysate.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab176851 was affinity purified using an epitope specific to DNMBP immobilized on solid support.
Our Abpromise guarantee covers the use of ab176851 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 177 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionScaffold protein that links dynamin with actin-regulating proteins. May play a role in membrane trafficking between the cell surface and the Golgi.
Tissue specificityDetected in heart, brain, lung, liver, skeletal muscle, kidney and pancreas.
Sequence similaritiesContains 1 BAR domain.
Contains 1 DH (DBL-homology) domain.
Contains 6 SH3 domains.
Cellular localizationCytoplasm. Golgi apparatus > Golgi stack. Cytoplasm > cytoskeleton. Cell junction > synapse. Localized to synapses and Golgi stacks.
- Information by UniProt
- Dnmbp antibody
- DNMBP_HUMAN antibody
- Dynamin binding protein antibody
All lanes : Anti-DNMBP antibody (ab176851) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 177 kDa
Exposure time: 30 seconds
Detection of DNMBP in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab176851 at 6 µg/mg lysate for IP (Lane 1). For WB ab176851 used at 0.4 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 3 seconds.
ab176851 has not yet been referenced specifically in any publications.