Overview

  • Product name
  • Description
    Rabbit polyclonal to DNMBP
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cynomolgus monkey, Rhesus monkey, Orangutan
  • Immunogen

    Synthetic peptide within Human DNMBP aa 575-625. The exact sequence is proprietary. NP_056036.1
    Sequence:

    RHFSIM DFNSEKDIVR GSSKLITEQE LPERRKALRP PPPRPCTPVS TSPHL


    Database link: Q6XZF7

  • Positive control
    • HeLa whole cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituents: 99% Tris buffered saline, 0.1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab176851 was affinity purified using an epitope specific to DNMBP immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab176851 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 177 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function
    Scaffold protein that links dynamin with actin-regulating proteins. May play a role in membrane trafficking between the cell surface and the Golgi.
  • Tissue specificity
    Detected in heart, brain, lung, liver, skeletal muscle, kidney and pancreas.
  • Sequence similarities
    Contains 1 BAR domain.
    Contains 1 DH (DBL-homology) domain.
    Contains 6 SH3 domains.
  • Cellular localization
    Cytoplasm. Golgi apparatus > Golgi stack. Cytoplasm > cytoskeleton. Cell junction > synapse. Localized to synapses and Golgi stacks.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dnmbp antibody
    • DNMBP_HUMAN antibody
    • Dynamin binding protein antibody
    • Dynamin-binding protein antibody
    • KIAA1010 antibody
    • Scaffold protein Tuba antibody
    • TUBA antibody
    see all

Images

  • All lanes : Anti-DNMBP antibody (ab176851) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique.

    Predicted band size: 177 kDa


    Exposure time: 30 seconds
  • Detection of DNMBP in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab176851 at 6 µg/mg lysate for IP (Lane 1). For WB ab176851 used at 0.4 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 3 seconds.

References

ab176851 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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