Recombinant
RabMAb

Anti-DNMBP antibody [EPR10015] (ab154836)

Overview

  • Product name
    Anti-DNMBP antibody [EPR10015]
    See all DNMBP primary antibodies
  • Description
    Rabbit monoclonal [EPR10015] to DNMBP
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human DNMBP (Uniprot: Q6XZF7)

  • Positive control
    • MCF7, Caco 2, HeLa and K562 cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20ºC.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR10015
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154836 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 177 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function
      Scaffold protein that links dynamin with actin-regulating proteins. May play a role in membrane trafficking between the cell surface and the Golgi.
    • Tissue specificity
      Detected in heart, brain, lung, liver, skeletal muscle, kidney and pancreas.
    • Sequence similarities
      Contains 1 BAR domain.
      Contains 1 DH (DBL-homology) domain.
      Contains 6 SH3 domains.
    • Cellular localization
      Cytoplasm. Golgi apparatus > Golgi stack. Cytoplasm > cytoskeleton. Cell junction > synapse. Localized to synapses and Golgi stacks.
    • Information by UniProt
    • Database links
    • Alternative names
      • Dnmbp antibody
      • DNMBP_HUMAN antibody
      • Dynamin binding protein antibody
      • Dynamin-binding protein antibody
      • KIAA1010 antibody
      • Scaffold protein Tuba antibody
      • TUBA antibody
      see all

    Images

    • All lanes : Anti-DNMBP antibody [EPR10015] (ab154836) at 1/1000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : Caco 2 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 177 kDa

    References

    ab154836 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Western blot
    Loading amount
    500 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Xenopus laevis Cell lysate - whole cell (Stage 11 Embryos)
    Specification
    Stage 11 Embryos
    Blocking step
    KPL Blocking Reagent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted Aug 13 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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