RecombinantRabMAb

Recombinant Anti-DNMBP antibody [EPR10015] (ab154836)

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Western blot - Anti-DNMBP antibody [EPR10015] (ab154836)
  • Anti-DNMBP antibody [EPR10015] (ab154836)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10015] to DNMBP
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-DNMBP antibody [EPR10015]
    See all DNMBP primary antibodies

  • Description

    Rabbit monoclonal [EPR10015] to DNMBP

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide corresponding to residues in Human DNMBP (Uniprot: Q6XZF7)

  • Positive control

    • MCF7, Caco 2, HeLa and K562 cell lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20ºC.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR10015

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154836 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 177 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

    • Target

    • Function

      Scaffold protein that links dynamin with actin-regulating proteins. May play a role in membrane trafficking between the cell surface and the Golgi.

    • Tissue specificity

      Detected in heart, brain, lung, liver, skeletal muscle, kidney and pancreas.

    • Sequence similarities

      Contains 1 BAR domain.
      Contains 1 DH (DBL-homology) domain.
      Contains 6 SH3 domains.

    • Cellular localization

      Cytoplasm. Golgi apparatus > Golgi stack. Cytoplasm > cytoskeleton. Cell junction > synapse. Localized to synapses and Golgi stacks.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Dnmbp antibody
      • DNMBP_HUMAN antibody
      • Dynamin binding protein antibody
      • Dynamin-binding protein antibody
      • KIAA1010 antibody
      • Scaffold protein Tuba antibody
      • TUBA antibody
      see all

    Images

    • Western blot - Anti-DNMBP antibody [EPR10015] (ab154836)
      Western blot - Anti-DNMBP antibody [EPR10015] (ab154836)
      All lanes : Anti-DNMBP antibody [EPR10015] (ab154836) at 1/1000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : Caco 2 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 177 kDa

    • Anti-DNMBP antibody [EPR10015] (ab154836)
      Anti-DNMBP antibody [EPR10015] (ab154836)

    References (0)

    Publishing research using ab154836? Please let us know so that we can cite the reference in this datasheet.

    ab154836 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Western blot abreview for Anti-DNMBP antibody [EPR10015]

     Average
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    500 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Xenopus laevis Cell lysate - whole cell (Stage 11 Embryos)
    Specification
    Stage 11 Embryos
    Blocking step
    KPL Blocking Reagent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 13 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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