Recombinant
RabMAb

Recombinant Anti-DNMBP antibody [EPR10015] (ab154836)

Reviews (1) Submit a question

Overview

  • Product name

    Anti-DNMBP antibody [EPR10015]
    See all DNMBP primary antibodies

  • Description

    Rabbit monoclonal [EPR10015] to DNMBP

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide corresponding to residues in Human DNMBP (Uniprot: Q6XZF7)

  • Positive control

    • MCF7, Caco 2, HeLa and K562 cell lysates.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20ºC.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR10015

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154836 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 177 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

    • Target

    • Function

      Scaffold protein that links dynamin with actin-regulating proteins. May play a role in membrane trafficking between the cell surface and the Golgi.

    • Tissue specificity

      Detected in heart, brain, lung, liver, skeletal muscle, kidney and pancreas.

    • Sequence similarities

      Contains 1 BAR domain.
      Contains 1 DH (DBL-homology) domain.
      Contains 6 SH3 domains.

    • Cellular localization

      Cytoplasm. Golgi apparatus > Golgi stack. Cytoplasm > cytoskeleton. Cell junction > synapse. Localized to synapses and Golgi stacks.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Dnmbp antibody
      • DNMBP_HUMAN antibody
      • Dynamin binding protein antibody
      • Dynamin-binding protein antibody
      • KIAA1010 antibody
      • Scaffold protein Tuba antibody
      • TUBA antibody
      see all

    Images

    • Western blot - Anti-DNMBP antibody [EPR10015] (ab154836)
      Western blot - Anti-DNMBP antibody [EPR10015] (ab154836)
      All lanes : Anti-DNMBP antibody [EPR10015] (ab154836) at 1/1000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : Caco 2 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 177 kDa

    References

    ab154836 has not yet been referenced specifically in any publications.

    Publishing research using ab154836? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot abreview for Anti-DNMBP antibody [EPR10015]

     Average
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    500 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Xenopus laevis Cell lysate - whole cell (Stage 11 Embryos)
    Specification
    Stage 11 Embryos
    Blocking step
    KPL Blocking Reagent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 13 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com