The kit is easy to use and contains all components needed to perform the colorimetric ELISA. Mouse brain samples were used with this kit. Nuclear extract was prepared (with a non abcam kit) from the previous collected mouse brain samples. From each sample 15 µg of nuclear extract was used to perform the ELISA. From here on the abcam protocol was followed. The blank an positive controls were included, and the positive control had the highest signal in my study design. Between different individual animals there was a low variation, speaking for the high sensivity and reproducibility of the kit.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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Submitted Jun 07 2013