Question (38889) | Anti-Dnmt1 antibody [60B1220.1] - ChIP Grade (ab13537)

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Question

1X CPE buffer is as follows:
20mM MOPS
62mM Sucrose
0.1 mM EDTA
1x Lysis buffer with protease inhibitors:
10 mM Tris
0.15 M NaCl
5mM EDTA
1% Triton X
Protease inhibitors for lysis buffer
1mM PMSF
25mg/ml leupeptin
25 ug/ml aprotinin
also sending the attached images as requested.
Thanks so much for your help.

Answer

Thanks for re-sending your western blot images and for providing the extraction buffer information.
I see what you are saying. Interestingly, it seems there is background staining in some but not all of the murine hepatocyte samples. Are there any differences in the samples that might explain this?

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