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1X CPE buffer is as follows:
0.1 mM EDTA
1x Lysis buffer with protease inhibitors:
10 mM Tris
0.15 M NaCl
1% Triton X
Protease inhibitors for lysis buffer
25 ug/ml aprotinin
also sending the attached images as requested.
Thanks so much for your help.
Asked on Mar 06 2012
Thanks for re-sending your western blot images and for providing the extraction buffer information.
I see what you are saying. Interestingly, it seems there is background staining in some but not all of the murine hepatocyte samples. Are there any differences in the samples that might explain this?
Answered on Mar 06 2012