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I am waiting for your answer for the antibody! I need to address reviewer's question so we are busy for lab experiments now.
Asked on Nov 21 2012
Thank you for your email.
We have decided to send you a replacement antibody. The antibody ab92453 is of same clone and same immunogen sequence. Could you check you and confirm?
Please note, although ab13537 and ab92453 seems same however they are developed for us by different laboratories so we can expect some variations as different preparations of the hybridoma are known to alter epitope specificity slightly.
Please also note that as of ab13537 this antibody has also not been tested in double staining where the second antibody was against same antigen. So protocol optimizations are highly recommended.
I will look forward to hearing from you soon. Please do not hesitate to contact me if you have any question.
Answered on Nov 21 2012