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I ordered your antibody 1 x 100µg : Dnmt1 antibody (ab19905) (number: 102003 received on 20-October-2006, our order ref: 184005). I read the information of this antibody at web site, but I could not find the concentlation of Tris EDTA which is used as antigen retrieval solution. Could you teach me molar concentration of Tris and EDTA.
Asked on Oct 30 2006
Thank you for your enquiry. The antigen retrieval solution that was used for the testing of rabbit polyclonal to Dnmt1 (ab19905) was Tris-EDTA pH9. This was a standard preparation as follows; Tris-EDTA Buffer (10mM Tris Base, 1mM EDTA Solution, 0.05% Tween 20, pH 9.0). For further details please see http://www.ihcworld.com/_protocols/epitope_retrieval/tris_edta.htm I hope this information helps, please do not hesitate to contact us if you need any more advice or information.
Answered on Nov 01 2006