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To whom it may concern,
I would like to ask you information regarding DNMT antibodies from yours. We are trying to purchase DNMT1, 3a, 3b antibodies, and we see there are several antibodies for same targets.
So, we will do WB, ChIP, and maybe IHC using those antibodies, and we see two good antibodies such as DNMT1 antibody (ab87656 and ab19905). ab87656 is applicable for ChIP, but it has worse review for WB. And ab19905 is not applicable for ChIP, but it has a better review and more publication references. It has all same issues with other antibodies too (DNMT3a, DNMT3b).
Could you recommend how we should purchase and use properly? If we are going to do ChIP, would it better to buy ChIP Grade antibody and do WB as well?
Asked on Oct 15 2012
Thank you for contacting us.
We will guarantee our antibodies for any species and applications listed as tested on our datasheets, even if the reviews are negative. If for any reason you are unsatisfied with your results when using one of our antibodies in a tested species or application, we are more than happy to refund or replace it for you.
If you are interested in testing any of our antibodies in an untested application, you may qualify for our Abtrial program:
If you purchase the antibody for full price and submit an Abreview with your protocol and results, we will provide a discount code that can be redeemed for your original purchase price off of a future order.
In general, ChIP tends to be the more sensitive application, and antibodies that work in ChIP will be more likely to work in WB than the other way around. I hope this helps, please let me know if you need any additional information or assistance.
Answered on Oct 15 2012