Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Dnmt1 antibody

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Testis)
Specification
Testis
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mmol/L citrate buffer, pH 6.0
Permeabilization
No
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 8% · Temperature: 37°C

Other product details

Dilution
1/100
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS

Secondary antibody

Name
Non-Abcam antibody was used: Biotinylated Anti-Rabbit IgG (H+L) Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/400

Additional data

Abcam response
Many thanks for your valuable feedback. The immunogen used to generate this antibody has 71% identity with the corresponding region in mouse Dnmt1. We have had variable feedback about the ability of this antibody to recognise mouse Dnmt1.

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Submitted Mar 22 2010

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