Western blot abreview for Anti-Dnmt1 antibody

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Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Amphopack)
Loading amount
20 µg
Specification
Amphopack
Gel Running Conditions
Reduced Denaturing
Blocking step
Li-Core Blocking reagent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0% · Temperature: 25°C

Other product details

Dilution
1/1000
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Li-Core Blocking reagent

Secondary antibody

Name
Non-Abcam antibody was used: Li-Core goat anti rabbit
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor® 488

Detection

Detection method
LiCore
Exposure
10 second(s)
Bands
Specific: 183 kDa

Additional data

Additional Notes
Lovely Antibody!

Cell source: AmphoPack-293 Human Cell line from Clonetech

Lane 1: Whole Cell Lysate
Lane 2: Whole Cell Lysate

Lower band is Actin loading control.

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Submitted Mar 28 2011

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