Western blot abreview for Anti-Dnmt1 antibody

Average
Abreviews
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (Gradient Gel)
Sample
Human Cell lysate - nuclear (HCT116)
Specification
HCT116
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C

Other product details

Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS + 0.5% Tween + Azide
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: anti-rabbit
Host species: Mouse
Clonality: Polyclonal
Conjugation: Other
Dilution
1/5000

Detection

Detection method
Odyssey machine
Bands
Specific: 185 kDa
Exposure
1 minute(s) and 0 second(s)

Additional data

Additional Notes
The lower bands were not observed, because the gel was allowed to run so that the protein 50KDa and lower escaped, for better resolution of bigger proteins.

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Submitted Dec 23 2013

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