Western blot abreview for Anti-Dnmt1 antibody

Good
Abreviews
Application
Western blot
Loading amount
100 µg
Gel Running Conditions
Reduced Denaturing (Gradient Gel)
Sample
Mouse Tissue lysate - whole (mouse ES cells)
Specification
mouse ES cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS + 0.5% Tween + Azide
Dilution
1/500

Secondary antibody

Name
Non-Abcam antibody was used: anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Other

Detection

Detection method
odyssey machine
Bands
Specific: 183 kDa
Exposure
1 minute(s) and 0 second(s)

Additional data

Abcam response
We appreciate the data provided. Since cross reactivity with murine samples seems to be lot specific for this antibody we prefer to not add this species to the list of tested species.

Abcam user community

Verified customer

Submitted Mar 03 2014

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