Overview

  • Product name
  • Description
    Rabbit polyclonal to Dnmt1
  • Host species
    Rabbit
  • Specificity
    Dnmt 1. Will recognize the splice isoforms B and C.
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to within the last 100 amino acids of Human Dnmt 1.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    Purified through a protein G column and eluted out with both high and low pH buffers, neutralized immediately after elution, followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab4870 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 183 kDa.

Target

  • Function
    Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9.
  • Tissue specificity
    Ubiquitous; highly expressed in fetal tissues, heart, kidney, placenta, peripheral blood mononuclear cells, and expressed at lower levels in spleen, lung, brain, small intestine, colon, liver, and skeletal muscle. Isoform 2 is less expressed than isoform 1.
  • Sequence similarities
    Belongs to the C5-methyltransferase family.
    Contains 2 BAH domains.
    Contains 1 CXXC-type zinc finger.
  • Domain
    The N-terminal part is required for homodimerization and acts as a regulatory domain.
  • Post-translational
    modifications
    Sumoylated; sumoylation increases activity.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADCADN antibody
    • AIM antibody
    • CXXC finger protein 9 antibody
    • CXXC-type zinc finger protein 9 antibody
    • CXXC9 antibody
    • DNA (cytosine 5 ) methyltransferase 1 antibody
    • DNA (cytosine-5)-methyltransferase 1 antibody
    • DNA methyltransferase 1 antibody
    • DNA methyltransferase HsaI antibody
    • DNA methyltransferase M.HsaI. antibody
    • DNA MTase antibody
    • DNA MTase HsaI antibody
    • DNMT 1 antibody
    • DNMT antibody
    • Dnmt1 antibody
    • DNMT1_HUMAN antibody
    • Dnmt1o antibody
    • FLJ16293 antibody
    • HSN1E antibody
    • M.HsaI antibody
    • MCMT antibody
    • Met1 antibody
    • MGC104992 antibody
    • mMmul antibody
    • MommeD2 antibody
    see all

Images

  • ab4870 at a 1:100 dilution staining approximately 180 kDa Dnmt 1 in Jurkat cell lysate by Western blot (ECL).

    ab4870 used in Western blot to detect Dnmt1 in Jurkat cell lysate.

References

This product has been referenced in:
  • Sarkar S  et al. Histone Deacetylase Inhibitors Reverse CpG Methylation by Regulating DNMT1 through ERK Signaling. Anticancer Res 31:2723-32 (2011). WB ; Human . Read more (PubMed: 21868513) »
See 1 Publication for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Answer

Thank you for your reply.

I am not sure what the lower bands might represent. They are a bit low to represent any of the isoforms of DNMT1 in rat. A BLAST of the immunogen sequence did not reveal any homology with another protein that may be detected by this antibody. It would be interesting to see how the results compare to human samples (positive control). We have not specifically tested the antibody in rat and thus we have not performed any further analysis with this sample type.


Please do not hesitate to contact me if you have any additional questions.

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Answer

Thank you for contacting Abcam.

I am sorry this product did not perform in rat as you had hoped and for the inconvenience this has caused. Since this antibody has not been tested in rat, we do not know if and how well it might work in this sample.

Unfortunately we do not have an antibody that has been specifically validated for rat, but I would like to recommend ab109981 as a potential alternative. This antibody is also predicted to work in rat and may be more suitable for your experiments.

Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Cultured Hepatoma Cell Line)
Specification
Cultured Hepatoma Cell Line
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Triton-X100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 27 2009

Abcam has not validated the combination of species/application used in this Abreview.
Application
ChIP
Sample
Rat Cell lysate - whole cell (Liver)
Specification
Liver
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 0 second(s)
Specification of the cross-linking agent: formaldehyde
Detection step
Real-time PCR
Positive control
ab5208 DNMT1 ChIP
Negative control
Rabbit IgG

Abcam user community

Verified customer

Submitted Nov 27 2006

Question

I have been struggling with your Dnmt1 antibodies. 1. Ab2849 seems to work well for immunofluorescence (in the bovine) but gives me about 10 spare bands on the western human and bovine(1:1000 incubation 4C ON and blocking 5% skim milk). Those bands are not due to binding of the secondary antibody since I`ve tested it and different concentrations of the primary doesnt seem to help getting rid of those bands. The biggest problem is that the antibody binds the spare bands at a higher intensity than it does the band with the right predicted size. Did you ever characterize/sequence the bands you identified by western? I ran 2D transfers (westerns) using cell extracts of mcf-7 (human mammarian tumor cell line) and was unable to detect a spot at the right size and PI (180-190kDa and pI 7.4-8.0) instead I detected high molecular weight bands at pI 4-6 which are not Dnmts. 2. Ab4870 seems to be more specific (fewer spare bands) however it is very very weak! It does not work well on westerns. Both antibodies were used for immunoprecipitation and did not give me a band at the right predicted size. 3. Ab 14290 - you claim it works in the bovine. How has it been tested? Do you have protein sequence data proving it? If you do would you be able to send us a sample of it or trade a vial of the Ab4870 for one of the Ab1420. As you can see we have spent a lot of money and time buying and testing your antibodies so I would appreciate if we could get some technical support. Last time I contacted your company I was not able to get much information. All I got was a general protocol for westerns.

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Answer

Thank you for your patience and I'm sorry to hear that you are experiencing difficulty with these antibodies. All the information that we have regarding these antibodies is located on the online datasheets; as these three antibodies were originated outside Abcam, I'm currently trying to obtain some more information from the originators to help you out. What were the lot numbers (located on the vials) that you received? What was the Abcam order number or purchase order number that was used? For ab4870, what were the dilutions that you tried and what type of samples did you use? Neither ab2849 or ab4870 have been tested before in IP, so there is no information regarding the use of the antibodies in that particular application.

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Question
Answer

The peptide sequence used as the immunogen is CSASAKIKEEEAAKD corresponding to the C-term of human Dnmt1 (NP_001370).

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Answer

The immunogen is a synthetic peptide conjugated to KLH, corresponding to within the last 100 amino acids of Human Dnmt 1. From analysing this sequence using Swiss Prot and BLAST I can see there is a 97% similarity between the human sequence and bovine.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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