• Product name
  • Description
    Rabbit polyclonal to Dnmt1
  • Host species
  • Specificity
    ab87656 is recommended for DNMT1 detection in WB.
  • Tested applications
    Suitable for: IP, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human, Xenopus laevis
  • Immunogen

    Highly purified recombinant Mouse Dnmt1 (amino acids 1-248), soluble form

  • Positive control
    • WB: HeLa cell nuclear extracts.
  • General notes

    Useful for analysis of the protein complex containing Dnmt1.


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: 50% Glycerol, traces of Ammonium sulphate, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Primary antibody notes
    Useful for analysis of the protein complex containing Dnmt1.
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab87654 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent dilution. 1 ug of the antibody can precipitates 0.5~1.0 ug of Dnmt1. Antibody works best for native Dnmt1.
ICC/IF 1/5000.
WB Use a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 183 kDa (predicted molecular weight: 183 kDa).


  • Function
    Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9.
  • Tissue specificity
    Ubiquitous; highly expressed in fetal tissues, heart, kidney, placenta, peripheral blood mononuclear cells, and expressed at lower levels in spleen, lung, brain, small intestine, colon, liver, and skeletal muscle. Isoform 2 is less expressed than isoform 1.
  • Sequence similarities
    Belongs to the C5-methyltransferase family.
    Contains 2 BAH domains.
    Contains 1 CXXC-type zinc finger.
  • Domain
    The N-terminal part is required for homodimerization and acts as a regulatory domain.
  • Post-translational
    Sumoylated; sumoylation increases activity.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • ADCADN antibody
    • AIM antibody
    • CXXC finger protein 9 antibody
    • CXXC-type zinc finger protein 9 antibody
    • CXXC9 antibody
    • DNA (cytosine 5 ) methyltransferase 1 antibody
    • DNA (cytosine-5)-methyltransferase 1 antibody
    • DNA methyltransferase 1 antibody
    • DNA methyltransferase HsaI antibody
    • DNA methyltransferase M.HsaI. antibody
    • DNA MTase antibody
    • DNA MTase HsaI antibody
    • DNMT 1 antibody
    • DNMT antibody
    • Dnmt1 antibody
    • DNMT1_HUMAN antibody
    • Dnmt1o antibody
    • FLJ16293 antibody
    • HSN1E antibody
    • M.HsaI antibody
    • MCMT antibody
    • Met1 antibody
    • MGC104992 antibody
    • mMmul antibody
    • MommeD2 antibody
    see all


  • ab87654 being used to indicate the subcellular localization of Dnmt1 in mouse embryonic stem cells (E14) during cell cycle progression.
  • All lanes : Anti-Dnmt1 antibody (ab87654) at 1 µg/ml

    Lane 1 : Tagged-NP 95 + BirA Input
    Lane 2 : Tagged-NP 95 + BirA Beads
    Lane 3 : BirA Input
    Lane 4 : BirA Beads

    Predicted band size: 183 kDa
    Observed band size: 183 kDa

    Association of Np95 with Dnmt1 and Pcna in HeLa cell nuclear extracts. Human NP95 was tagged by biotin-binding domain and stably expressed in HeLa cells together with the E. coli BirA biotin ligase gene. Biotylated Np95 was captured by streptoavidin beads. The captured proteins were eluted from the beads and analyzed by western bloting using antibodies against Dnmt1 (ab87654) and Pcna.
  • All lanes : Anti-Dnmt1 antibody (ab87654) at 1 µg/ml

    Lane 1 : Dnmt1 1+/+ (J1) (nuclear extract)
    Lane 2 : Dnmt1 1-/- (36-2D6) (nuclear extract)
    Lane 3 : Tagged-NP95 + BirA (0.1M KCl fraction)
    Lane 4 : BirA (0.1M KCl fraction)

    Predicted band size: 183 kDa

    Catalytic activity of NP95 associated Dnmt1 in HeLa cells. The presence of Dnmt1 in the NP95 complexes was confirmed by western blot.


This product has been referenced in:
  • Shi H  et al. miR-148a suppresses cell invasion and migration in gastric cancer by targeting DNA methyltransferase 1. Oncol Lett 15:4944-4950 (2018). Read more (PubMed: 29541249) »
  • Zhou FC  et al. Cell-Wide DNA De-Methylation and Re-Methylation of Purkinje Neurons in the Developing Cerebellum. PLoS One 11:e0162063 (2016). IHC-FrFl ; Mouse . Read more (PubMed: 27583369) »

See all 7 Publications for this product

Customer reviews and Q&As

The cell extract, containinjg about 10 ug protein, is applied to each lane in SDS-PAGE and 0.2˜1.0 ug/ml of the antibody are used in WB. There is no special protocol needed for using this antibody. However, it should be kept in mind that Dnmt1 protein ...

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Thank you for contacting us.

I am sorry to hear that the antibody is not working as expected.

As we discussed over the phone, please let me or my colleague xxx know if the 1/5000 dilution and using mouse samples improved your resul...

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Thank you for contacting us. I am waiting for confirmation from the laboratory to confirm that the Dnmt1 antibody has been tested on Hela by ICC and will send this to you when I receive it, assuming it exists. If there is no data, I do still think it w...

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