Western blot abreview for Anti-Dnmt1 antibody [EPR18453]

Excellent
Abreviews
Application
Western blot
Sample
Chicken Cell lysate - whole cell (Chincken B cell line DT40)
Gel Running Conditions
Reduced Denaturing (3-8% Tris-acetate)
Loading amount
100000 cells
Specification
Chincken B cell line DT40
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Other product details

Incubation time
3 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 1 x PBS + 0.2% Tween-20
Dilution
1/1000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: anti-rabbit IgG (H+L) Secondary Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
regular ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 183 kDa

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Submitted Apr 22 2016

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