• Product name

    Anti-Dnmt1 antibody [EPR3522]
    See all Dnmt1 primary antibodies
  • Description

    Rabbit monoclonal [EPR3522] to Dnmt1
  • Host species

  • Tested applications

    Suitable for: WB, IP, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Dnmt1 aa 1600-1700 (C terminal). The exact sequence is proprietary.

  • Positive control

    • HuT-78, Jurkat or 293T lysate HeLa cells
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.


  • Form

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

  • Clone number

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab92314 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 183 kDa.
IP 1/20.
Flow Cyt 1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.


ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9.
    • Tissue specificity

      Ubiquitous; highly expressed in fetal tissues, heart, kidney, placenta, peripheral blood mononuclear cells, and expressed at lower levels in spleen, lung, brain, small intestine, colon, liver, and skeletal muscle. Isoform 2 is less expressed than isoform 1.
    • Sequence similarities

      Belongs to the C5-methyltransferase family.
      Contains 2 BAH domains.
      Contains 1 CXXC-type zinc finger.
    • Domain

      The N-terminal part is required for homodimerization and acts as a regulatory domain.
    • Post-translational

      Sumoylated; sumoylation increases activity.
    • Cellular localization

    • Information by UniProt
    • Database links

    • Alternative names

      • ADCADN antibody
      • AIM antibody
      • CXXC finger protein 9 antibody
      • CXXC-type zinc finger protein 9 antibody
      • CXXC9 antibody
      • DNA (cytosine 5 ) methyltransferase 1 antibody
      • DNA (cytosine-5)-methyltransferase 1 antibody
      • DNA methyltransferase 1 antibody
      • DNA methyltransferase HsaI antibody
      • DNA methyltransferase M.HsaI. antibody
      • DNA MTase antibody
      • DNA MTase HsaI antibody
      • DNMT 1 antibody
      • DNMT antibody
      • Dnmt1 antibody
      • DNMT1_HUMAN antibody
      • Dnmt1o antibody
      • FLJ16293 antibody
      • HSN1E antibody
      • M.HsaI antibody
      • MCMT antibody
      • Met1 antibody
      • MGC104992 antibody
      • mMmul antibody
      • MommeD2 antibody
      see all


    • Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: DNMT1 knockout  HAP1 whole cell lysate (20 µg)
      Lane 3: HEK293 whole cell lysate (20 µg)
      Lane 4: HeLa whole cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab92314 observed at 170 kDa. Red - loading control, ab18058, observed at 130 kDa.

      ab92314 was shown to specifically react with DNMT1 when DNMT1 knockout samples were used. Wild-type and DNMT1 knockout samples were subjected to SDS-PAGE.  Ab92314 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.


    • Immunofluorescence staining of Jurkat cells with purified ab92314 at a working dilution of 1/2000, counter-stained with DAPI. The secondary antibody was an Alexa Fluor® 488 conjugated goat anti-rabbit (ab150077), used at a dilution of 1/1000. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, PBS was used instead of the primary antibody.

    • Overlay histogram showing HeLa cells stained with ab92314 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92314, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • All lanes : Anti-Dnmt1 antibody [EPR3522] (ab92314) at 1/1000 dilution

      Lane 1 : HuT-78 cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 183 kDa


    This product has been referenced in:

    • Molokie R  et al. Oral tetrahydrouridine and decitabine for non-cytotoxic epigenetic gene regulation in sickle cell disease: A randomized phase 1 study. PLoS Med 14:e1002382 (2017). Read more (PubMed: 28880867) »
    • Heuslein JL  et al. DNA Methyltransferase 1-Dependent DNA Hypermethylation Constrains Arteriogenesis by Augmenting Shear Stress Set Point. J Am Heart Assoc 6:N/A (2017). Read more (PubMed: 29191807) »
    See all 5 Publications for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A


    Thank you for your inquiry.

    I have been in touch with the lab and they can provide custom conjugation of this antibody to biotin. They do require about a 1mg order. Did you have a quantity in mind so that I can get a quote from the lab?

    Please let me know and we look forward to helping you further.

    Read More


    Thank you for contacting us. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number ***. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

    Read More


    Thank you for contacting us and for sending an image of your staining. Our Abpromise Guarantee covers all of our products for the tested species and applications listed on our datasheet for six months from the date of purchase. In the application notes on our datasheet, we do note that ab92314 has been found to be unsuitable for IHC-P. We have not validated it for use in any other forms of tissue staining such as IHC-Fr. If you could please send me some details of the protocol you used, I would be happy to offer suggestions to help improve your results. For example, how were the samples fixed and embedded? Did you perform any antigen retrieval? What blocking agent was used? What were the primary and secondary antibody concentrations and incubation times? Alternatively, if you have obtained unsatisfactory results using this antibody in a validated application such as Flow Cytometry, ICC, IP, or WB, please let me know. If you send me the results and protocol used for a tested application, I would be happy to offer you a free of charge replacement, credit, or refund. I hope this helps, please let me know if you need any additional assistance.

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