Anti-Dnmt2 antibody [ABM1H70] (ab220175)
Key features and details
- Mouse monoclonal [ABM1H70] to Dnmt2
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-Dnmt2 antibody [ABM1H70]
See all Dnmt2 primary antibodies -
Description
Mouse monoclonal [ABM1H70] to Dnmt2 -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human Dnmt2.
Database link: Q6ICS7 -
Positive control
- Jurkat and mouse embryonic liver lysates; HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
ABM1H70 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab220175 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 4 - 6 µg/ml. Predicted molecular weight: 45 kDa.
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Flow Cyt |
Use 0.5-1µg for 106 cells.
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Notes |
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WB
Use a concentration of 4 - 6 µg/ml. Predicted molecular weight: 45 kDa. |
Flow Cyt
Use 0.5-1µg for 106 cells. |
Target
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Function
Specifically methylates cytosine 38 in the anticodon loop of tRNA(Asp). -
Tissue specificity
Ubiquitous. Higher expression in testis, ovary and thymus and at much lower levels in spleen, prostate, colon, small intestine, and peripheral blood leukocytes. -
Sequence similarities
Belongs to the C5-methyltransferase family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 1787 Human
- Entrez Gene: 13434 Mouse
- Omim: 602478 Human
- SwissProt: O14717 Human
- SwissProt: O55055 Mouse
- Unigene: 351665 Human
- Unigene: 6979 Mouse
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Alternative names
- dDNMT antibody
- DmMT 2 antibody
- DmMT2 antibody
see all
Images
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Flow cytometric analysis of HeLa cells (4% paraformaldehyde-fixed and permeabilized) labeling Dnmt2 with ab220175 at 0.5 μg per 106 cells, followed by goat anti mouse PE conjugated secondary detection (red). Isotype control: green.
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All lanes : Anti-Dnmt2 antibody [ABM1H70] (ab220175) at 4 µg/ml
Lane 1 : Jurkat cell lysate
Lane 2 : mouse embryonic liver lysate
Predicted band size: 45 kDa
Datasheets and documents
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Datasheet download
References (1)
ab220175 has been referenced in 1 publication.
- Geng Q et al. Comprehensive Analysis of the Prognostic Value and Immune Infiltrates of the Three-m5C Signature in Colon Carcinoma. Cancer Manag Res 13:7989-8002 (2021). PubMed: 34707405