Key features and details
- Mouse monoclonal [ABM1H70] to Dnmt2
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG1
Product nameAnti-Dnmt2 antibody [ABM1H70]
See all Dnmt2 primary antibodies
DescriptionMouse monoclonal [ABM1H70] to Dnmt2
Tested applicationsSuitable for: WB, Flow Cytmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human Dnmt2.
Database link: Q6ICS7
- Jurkat and mouse embryonic liver lysates; HeLa cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA
Concentration information loading...
PurityProtein G purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab220175 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 4 - 6 µg/ml. Predicted molecular weight: 45 kDa.|
|Flow Cyt||Use 0.5-1µg for 106 cells.|
FunctionSpecifically methylates cytosine 38 in the anticodon loop of tRNA(Asp).
Tissue specificityUbiquitous. Higher expression in testis, ovary and thymus and at much lower levels in spleen, prostate, colon, small intestine, and peripheral blood leukocytes.
Sequence similaritiesBelongs to the C5-methyltransferase family.
- Information by UniProt
- dDNMT antibody
- DmMT 2 antibody
- DmMT2 antibody
Flow cytometric analysis of HeLa cells (4% paraformaldehyde-fixed and permeabilized) labeling Dnmt2 with ab220175 at 0.5 μg per 106 cells, followed by goat anti mouse PE conjugated secondary detection (red). Isotype control: green.
All lanes : Anti-Dnmt2 antibody [ABM1H70] (ab220175) at 4 µg/ml
Lane 1 : Jurkat cell lysate
Lane 2 : mouse embryonic liver lysate
Predicted band size: 45 kDa
ab220175 has not yet been referenced specifically in any publications.