Overview

  • Product name

    Anti-Dnmt2 antibody [ABM1H70]
    See all Dnmt2 primary antibodies
  • Description

    Mouse monoclonal [ABM1H70] to Dnmt2
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human Dnmt2.
    Database link: Q6ICS7

  • Positive control

    • Jurkat and mouse embryonic liver lysates; HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    ABM1H70
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab220175 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 4 - 6 µg/ml. Predicted molecular weight: 45 kDa.
Flow Cyt Use 0.5-1µg for 106 cells.

Target

  • Function

    Specifically methylates cytosine 38 in the anticodon loop of tRNA(Asp).
  • Tissue specificity

    Ubiquitous. Higher expression in testis, ovary and thymus and at much lower levels in spleen, prostate, colon, small intestine, and peripheral blood leukocytes.
  • Sequence similarities

    Belongs to the C5-methyltransferase family.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • dDNMT antibody
    • DmMT 2 antibody
    • DmMT2 antibody
    • DNA (cytosine 5 ) methyltransferase 2 antibody
    • DNA (cytosine 5) methyltransferase like protein 2 antibody
    • DNA (cytosine-5)-methyltransferase-like protein 2 antibody
    • DNA 5 cytosine methyltransferase antibody
    • DNA methyltransferase 2 antibody
    • DNA methyltransferase homolog HsaIIP antibody
    • DNA MTase homolog HsaIIP antibody
    • Dnmt 2 antibody
    • Dnmt2 antibody
    • M.HsaIIP antibody
    • MHsaIIP antibody
    • nmt 2 antibody
    • nmt2 antibody
    • OTTHUMP00000045198 antibody
    • PuMet antibody
    • RNMT 1 antibody
    • RNMT1 antibody
    • TRDMT 1 antibody
    • TRDMT_HUMAN antibody
    • TRDMT1 antibody
    • tRNA (cytosine 5 ) methyltransferase antibody
    • tRNA (cytosine(38)-C(5))-methyltransferase antibody
    • tRNA aspartic acid methyltransferase 1 antibody
    • tRNA aspartic acid methyltransferase 1 variant 1 antibody
    • tRNA aspartic acid methyltransferase 1 variant 2 antibody
    • tRNA aspartic acid methyltransferase 1 variant 3 antibody
    • tRNA aspartic acid methyltransferase 1 variant 4 antibody
    • tRNA aspartic acid methyltransferase 1 variant 5 antibody
    • tRNA aspartic acid methyltransferase 1 variant 8 antibody
    see all

Images

  • Flow cytometric analysis of HeLa cells (4% paraformaldehyde-fixed and permeabilized) labeling Dnmt2 with ab220175 at 0.5 μg per 106 cells, followed by goat anti mouse PE conjugated secondary detection (red). Isotype control: green.

  • All lanes : Anti-Dnmt2 antibody [ABM1H70] (ab220175) at 4 µg/ml

    Lane 1 : Jurkat cell lysate
    Lane 2 : mouse embryonic liver lysate

    Predicted band size: 45 kDa

References

ab220175 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab220175.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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