Anti-Dnmt3a antibody (ab16704)
Key features and details
- Rabbit polyclonal to Dnmt3a
- Suitable for: WB, IP, EMSA
- Reacts with: Chicken
- Isotype: IgG
Overview
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Product name
Anti-Dnmt3a antibody
See all Dnmt3a primary antibodies -
Description
Rabbit polyclonal to Dnmt3a -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, EMSAmore details
Unsuitable for: Neut -
Species reactivity
Reacts with: Chicken -
Immunogen
Synthetic peptide, corresponding to amino acids 10-118 of Human Dnmt3a.
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Positive control
- HCT116 whole cell lysate.
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General notes
It is strongly suggested that Sodium Azide is added to this stock upon thawing and storage at 4 degrees C.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Constituent: Whole serum -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab16704 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | ||
| IP | ||
| EMSA |
Application notes
EMSA: 2ul antiserum is used in PMID 19046370.
IP: 1/1000.
WB: 1/1000. Detects a band of approximately 85 kDa.
Is unsuitable for Neut.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
IP: 1/1000.
WB: 1/1000. Detects a band of approximately 85 kDa.
Is unsuitable for Neut.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Required for genome wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZNF238. Can actively repress transcription through the recruitment of HDAC activity. -
Tissue specificity
Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung. -
Sequence similarities
Belongs to the C5-methyltransferase family.
Contains 1 ADD domain.
Contains 1 GATA-type zinc finger.
Contains 1 PHD-type zinc finger.
Contains 1 PWWP domain. -
Domain
The PWWP domain is essential for targeting to pericentric heterochromatin. -
Post-translational
modificationsSumoylated; sumoylation disrupts the ability to interact with histone deacetylases (HDAC1 and HDAC2) and repress transcription. -
Cellular localization
Nucleus. Cytoplasm. Accumulates in the major satellite repeats at pericentric heterochromatin. - Information by UniProt
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Database links
- Entrez Gene: 421991 Chicken
- SwissProt: Q4W5Z4 Chicken
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Alternative names
- DNA (cytosine 5) methyltransferase 3 alpha antibody
- DNA (cytosine 5) methyltransferase 3A antibody
- DNA (cytosine-5)-methyltransferase 3A antibody
see all
Protocols
Datasheets and documents
References
ab16704 has been referenced in 5 publications.
- Abdalla BA et al. A Novel Dnmt3a1 Transcript Inhibits Adipogenesis. Front Physiol 9:1270 (2018). PubMed: 30333755
- Yossifoff M et al. Dynamic changes in DNA methylation during thermal control establishment affect CREB binding to the brain-derived neurotrophic factor promoter. Eur J Neurosci 28:2267-77 (2008). WB, EMSA ; Chicken . PubMed: 19046370
- Loriot A et al. Transient down-regulation of DNMT1 methyltransferase leads to activation and stable hypomethylation of MAGE-A1 in melanoma cells. J Biol Chem 281:10118-26 (2006). PubMed: 16497664
- Robert MF et al. DNMT1 is required to maintain CpG methylation and aberrant gene silencing in human cancer cells. Nat Genet 33:61-5 (2003). PubMed: 12496760
- Beaulieu N et al. An essential role for DNA methyltransferase DNMT3B in cancer cell survival. J Biol Chem 277:28176-81 (2002). PubMed: 12015329
