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    dnmt3a-antibody-ab16704.pdf

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Epigenetics and Nuclear Signaling DNA methylation DNMTs
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Anti-Dnmt3a antibody (ab16704)

  • Datasheet
Submit a review Submit a question References (6)

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Immunocytochemistry - Anti-Dnmt3a antibody (ab16704)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt3a antibody (ab16704)

Key features and details

  • Rabbit polyclonal to Dnmt3a
  • Suitable for: IHC-P, ICC
  • Reacts with: Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Dnmt3a antibody [EPR18455-94] (ab227823)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Dnmt3a antibody
    See all Dnmt3a primary antibodies
  • Description

    Rabbit polyclonal to Dnmt3a
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICCmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids 10-118 of Human Dnmt3a.

  • Positive control

    • ICC: Ntera-2 whole cell lysate. IHC-P: Human testis tissue
  • General notes

    It is strongly suggested that Sodium Azide is added to this stock upon thawing and storage at 4 degrees C.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Constituent: Whole serum
  • Concentration information loading...
  • Purity

    Whole antiserum
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA methylation
    • DNMTs

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human Dnmt3a protein (ab170408)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab16704 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/200.
ICC
1/500.
Notes
IHC-P
1/200.
ICC
1/500.

Target

  • Function

    Required for genome wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZNF238. Can actively repress transcription through the recruitment of HDAC activity.
  • Tissue specificity

    Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung.
  • Sequence similarities

    Belongs to the C5-methyltransferase family.
    Contains 1 ADD domain.
    Contains 1 GATA-type zinc finger.
    Contains 1 PHD-type zinc finger.
    Contains 1 PWWP domain.
  • Domain

    The PWWP domain is essential for targeting to pericentric heterochromatin.
  • Post-translational
    modifications

    Sumoylated; sumoylation disrupts the ability to interact with histone deacetylases (HDAC1 and HDAC2) and repress transcription.
  • Cellular localization

    Nucleus. Cytoplasm. Accumulates in the major satellite repeats at pericentric heterochromatin.
  • Target information above from: UniProt accession Q9Y6K1 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1788 Human
    • Omim: 602769 Human
    • SwissProt: Q9Y6K1 Human
    • Unigene: 515840 Human
    • Alternative names

      • DNA (cytosine 5) methyltransferase 3 alpha antibody
      • DNA (cytosine 5) methyltransferase 3A antibody
      • DNA (cytosine-5)-methyltransferase 3A antibody
      • DNA cytosine methyltransferase 3A2 antibody
      • DNA methyltransferase 3 alpha antibody
      • DNA methyltransferase 3a antibody
      • DNA methyltransferase HsaIIIA antibody
      • DNA MTase HsaIIIA antibody
      • DNM3A_HUMAN antibody
      • DNMT 3a antibody
      • DNMT antibody
      • Dnmt3a antibody
      • DNMT3A2 antibody
      • M.HsaIIIA antibody
      • MCMT antibody
      • OTTHUMP00000201149 antibody
      • TBRS antibody
      see all

    Images

    • Immunocytochemistry - Anti-Dnmt3a antibody (ab16704)
      Immunocytochemistry - Anti-Dnmt3a antibody (ab16704)
      Immunocytochemistry analysis of Ntera-2 cells labeling Dnmt3a with ab16704 followed by Dylight 488 (green). Nuclei and beta-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt3a antibody (ab16704)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt3a antibody (ab16704)

      Immunohistochemistry analysis of human testis labeling Dnmt3a with ab16704 at 1/200. Hematoxylin was used as counterstain.

       

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (6)

    Publishing research using ab16704? Please let us know so that we can cite the reference in this datasheet.

    ab16704 has been referenced in 6 publications.

    • Huang YH  et al. Systematic Profiling of DNMT3A Variants Reveals Protein Instability Mediated by the DCAF8 E3 Ubiquitin Ligase Adaptor. Cancer Discov N/A:N/A (2021). PubMed: 34429321
    • Abdalla BA  et al. A Novel Dnmt3a1 Transcript Inhibits Adipogenesis. Front Physiol 9:1270 (2018). PubMed: 30333755
    • Yossifoff M  et al. Dynamic changes in DNA methylation during thermal control establishment affect CREB binding to the brain-derived neurotrophic factor promoter. Eur J Neurosci 28:2267-77 (2008). WB, EMSA ; Chicken . PubMed: 19046370
    • Loriot A  et al. Transient down-regulation of DNMT1 methyltransferase leads to activation and stable hypomethylation of MAGE-A1 in melanoma cells. J Biol Chem 281:10118-26 (2006). PubMed: 16497664
    • Robert MF  et al. DNMT1 is required to maintain CpG methylation and aberrant gene silencing in human cancer cells. Nat Genet 33:61-5 (2003). PubMed: 12496760
    • Beaulieu N  et al. An essential role for DNA methyltransferase DNMT3B in cancer cell survival. J Biol Chem 277:28176-81 (2002). PubMed: 12015329

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