Overview

  • Product name
    Anti-Dnmt3a antibody
    See all Dnmt3a primary antibodies
  • Description
    Rabbit polyclonal to Dnmt3a
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, EMSAmore details
    Unsuitable for: Neut
  • Species reactivity
    Reacts with: Chicken, Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids 10-118 of Human Dnmt3a.

  • Positive control
    • HCT116 whole cell lysate.
  • General notes
    It is strongly suggested that Sodium Azide is added to this stock upon thawing and storage at 4 degrees C.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: Whole serum
  • Purity
    Whole antiserum
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab16704 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
EMSA
  • Application notes
    EMSA: 2ul antiserum is used in PMID 19046370.
    IP: 1/1000.
    WB: 1/1000. Detects a band of approximately 85 kDa.

    Is unsuitable for Neut.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Function
      Required for genome wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZNF238. Can actively repress transcription through the recruitment of HDAC activity.
    • Tissue specificity
      Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung.
    • Sequence similarities
      Belongs to the C5-methyltransferase family.
      Contains 1 ADD domain.
      Contains 1 GATA-type zinc finger.
      Contains 1 PHD-type zinc finger.
      Contains 1 PWWP domain.
    • Domain
      The PWWP domain is essential for targeting to pericentric heterochromatin.
    • Post-translational
      modifications
      Sumoylated; sumoylation disrupts the ability to interact with histone deacetylases (HDAC1 and HDAC2) and repress transcription.
    • Cellular localization
      Nucleus. Cytoplasm. Accumulates in the major satellite repeats at pericentric heterochromatin.
    • Information by UniProt
    • Database links
      • Alternative names
        • DNA (cytosine 5) methyltransferase 3 alpha antibody
        • DNA (cytosine 5) methyltransferase 3A antibody
        • DNA (cytosine-5)-methyltransferase 3A antibody
        • DNA cytosine methyltransferase 3A2 antibody
        • DNA methyltransferase 3 alpha antibody
        • DNA methyltransferase 3a antibody
        • DNA methyltransferase HsaIIIA antibody
        • DNA MTase HsaIIIA antibody
        • DNM3A_HUMAN antibody
        • DNMT 3a antibody
        • DNMT antibody
        • Dnmt3a antibody
        • DNMT3A2 antibody
        • M.HsaIIIA antibody
        • MCMT antibody
        • OTTHUMP00000201149 antibody
        • TBRS antibody
        see all

      References

      This product has been referenced in:
      • Yossifoff M  et al. Dynamic changes in DNA methylation during thermal control establishment affect CREB binding to the brain-derived neurotrophic factor promoter. Eur J Neurosci 28:2267-77 (2008). WB, EMSA ; Chicken . Read more (PubMed: 19046370) »
      • Loriot A  et al. Transient down-regulation of DNMT1 methyltransferase leads to activation and stable hypomethylation of MAGE-A1 in melanoma cells. J Biol Chem 281:10118-26 (2006). Read more (PubMed: 16497664) »

      See all 4 Publications for this product

      Publishing research using ab16704? Please let us know so that we can cite the reference in this datasheet.

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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