Anti-Dnmt3a antibody (ab2850)
Key features and details
- Rabbit polyclonal to Dnmt3a
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
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Overview
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Product name
Anti-Dnmt3a antibody
See all Dnmt3a primary antibodies -
Description
Rabbit polyclonal to Dnmt3a -
Host species
Rabbit -
Specificity
This antibody detects DNA methyltransferase 3a (Dnmt3a) from human and mouse tissues and cells as well as recombinant human Dnmt3a. This antibody does not detect full length recombinant human Dnmt3b or Dnmt1. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human Dnmt3a aa 50-150.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2850 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (4) |
Use a concentration of 2 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 101.5 kDa).
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Notes |
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WB
Use a concentration of 2 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 101.5 kDa). |
Target
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Function
Required for genome wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZNF238. Can actively repress transcription through the recruitment of HDAC activity. -
Tissue specificity
Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung. -
Sequence similarities
Belongs to the C5-methyltransferase family.
Contains 1 ADD domain.
Contains 1 GATA-type zinc finger.
Contains 1 PHD-type zinc finger.
Contains 1 PWWP domain. -
Domain
The PWWP domain is essential for targeting to pericentric heterochromatin. -
Post-translational
modificationsSumoylated; sumoylation disrupts the ability to interact with histone deacetylases (HDAC1 and HDAC2) and repress transcription. -
Cellular localization
Nucleus. Cytoplasm. Accumulates in the major satellite repeats at pericentric heterochromatin. - Information by UniProt
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Database links
- Entrez Gene: 1788 Human
- Entrez Gene: 13435 Mouse
- Omim: 602769 Human
- SwissProt: Q9Y6K1 Human
- SwissProt: O88508 Mouse
- Unigene: 515840 Human
- Unigene: 5001 Mouse
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Alternative names
- DNA (cytosine 5) methyltransferase 3 alpha antibody
- DNA (cytosine 5) methyltransferase 3A antibody
- DNA (cytosine-5)-methyltransferase 3A antibody
see all
Images
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Western blot analysis of DNMT3a in HeLa silenced cells. Total cell extracts were prepared 48 h after electroporation and analyzed by immunoblot with ab2850.
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Western blot of Dnmt3a on P19 cell nuclear extract using ab2850.
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All lanes : Anti-Dnmt3a antibody (ab2850) at 1/500 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HEK293 cell lysate
Lane 4 : A549 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L), Superclonal™ HRP at 1/1000 dilution
Predicted band size: 101.5 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
References (114)
ab2850 has been referenced in 114 publications.
- Tanabe R et al. PRRX1 induced by BMP signaling decreases tumorigenesis by epigenetically regulating glioma-initiating cell properties via DNA methyltransferase 3A. Mol Oncol 16:269-288 (2022). PubMed: 34214250
- Ren F et al. Iodine-125 seed represses the growth and facilitates the apoptosis of colorectal cancer cells by suppressing the methylation of miR-615 promoter. BMC Cancer 22:49 (2022). PubMed: 34998382
- Martínez-Iglesias O et al. AntiGan: An Epinutraceutical Bioproduct with Antitumor Properties in Cultured Cell Lines. Life (Basel) 12:N/A (2022). PubMed: 35054489
- Yan N et al. Developmental arsenic exposure impairs cognition, directly targets DNMT3A, and reduces DNA methylation. EMBO Rep 23:e54147 (2022). PubMed: 35373418
- Wang L et al. Dexmedetomidine prevents cardiomyocytes from hypoxia/reoxygenation injury via modulating tetmethylcytosine dioxygenase 1-mediated DNA demethylation of Sirtuin1. Bioengineered 13:9369-9386 (2022). PubMed: 35387565