Anti-Dnmt3a antibody (ab4897)
Key features and details
- Rabbit polyclonal to Dnmt3a
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Dnmt3a antibody
See all Dnmt3a primary antibodies -
Description
Rabbit polyclonal to Dnmt3a -
Host species
Rabbit -
Tested Applications & Species
Application Species IHC-P HumanWB Human -
Immunogen
Synthetic peptide corresponding to Human Dnmt3a aa 457-486 conjugated to Keyhole Limpet Haemocyanin (KLH).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Ammonium Sulphate Precipitation -
Purification notes
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab4897 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
---|---|
IHC-P |
Human
|
WB |
Human
|
All applications |
Mouse
Rat
Chicken
|
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/50 - 1/100.
|
|
WB |
1/1000. Detects a band of approximately 101 kDa.
|
Notes |
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IHC-P
1/50 - 1/100. |
WB
1/1000. Detects a band of approximately 101 kDa. |
Target
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Function
Required for genome wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZNF238. Can actively repress transcription through the recruitment of HDAC activity. -
Tissue specificity
Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung. -
Sequence similarities
Belongs to the C5-methyltransferase family.
Contains 1 ADD domain.
Contains 1 GATA-type zinc finger.
Contains 1 PHD-type zinc finger.
Contains 1 PWWP domain. -
Domain
The PWWP domain is essential for targeting to pericentric heterochromatin. -
Post-translational
modificationsSumoylated; sumoylation disrupts the ability to interact with histone deacetylases (HDAC1 and HDAC2) and repress transcription. -
Cellular localization
Nucleus. Cytoplasm. Accumulates in the major satellite repeats at pericentric heterochromatin. - Information by UniProt
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Database links
- Entrez Gene: 421991 Chicken
- Entrez Gene: 1788 Human
- Entrez Gene: 13435 Mouse
- Entrez Gene: 444984 Rat
- Omim: 602769 Human
- SwissProt: Q4W5Z4 Chicken
- SwissProt: Q9Y6K1 Human
- SwissProt: O88508 Mouse
see all -
Alternative names
- DNA (cytosine 5) methyltransferase 3 alpha antibody
- DNA (cytosine 5) methyltransferase 3A antibody
- DNA (cytosine-5)-methyltransferase 3A antibody
see all
Images
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Anti-Dnmt3a antibody (ab4897) + T47-D cell lysate
Secondary
HRP-conjugated anti-rabbit IgG -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt3a antibody (ab4897)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocarcinoma tissue labelling Dnmt3a with ab4897.
Protocols
Datasheets and documents
References (12)
ab4897 has been referenced in 12 publications.
- Yuan H et al. Hypomethylation of nerve growth factor (NGF) promotes binding of C/EBPa and contributes to inflammatory hyperalgesia in rats. J Neuroinflammation 17:34 (2020). PubMed: 31980031
- Li X et al. MCM3AP-AS1 KD Inhibits Proliferation, Invasion, and Migration of PCa Cells via DNMT1/DNMT3 (A/B) Methylation-Mediated Upregulation of NPY1R. Mol Ther Nucleic Acids 20:265-278 (2020). PubMed: 32193153
- Xu X et al. The long non-coding RNA Linc-GALH promotes hepatocellular carcinoma metastasis via epigenetically regulating Gankyrin. Cell Death Dis 10:86 (2019). PubMed: 30692513
- Akiyama N et al. Regulation of DNA methylation levels in the process of oral mucosal regeneration in a rat oral ulcer model. Histol Histopathol N/A:18147 (2019). PubMed: 31286466
- Ma M et al. Preeclampsia is associated with hypermethylation of IGF-1 promoter mediated by DNMT1. Am J Transl Res 10:16-39 (2018). IHC-P ; Human . PubMed: 29422991