Overview

  • Product name
    Anti-Dnmt3a antibody
    See all Dnmt3a primary antibodies
  • Description
    Rabbit polyclonal to Dnmt3a
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken
  • Immunogen

    Synthetic peptide corresponding to Human Dnmt3a aa 457-486 conjugated to Keyhole Limpet Haemocyanin (KLH).

  • Positive control
    • WB: T247-D cell lysate. IHC-P: Human hepatocarcinoma tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Ammonium Sulphate Precipitation
  • Purification notes
    This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab4897 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/400.
IHC-P 1/50 - 1/100.
WB 1/1000. Detects a band of approximately 101 kDa.

Target

  • Function
    Required for genome wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZNF238. Can actively repress transcription through the recruitment of HDAC activity.
  • Tissue specificity
    Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung.
  • Sequence similarities
    Belongs to the C5-methyltransferase family.
    Contains 1 ADD domain.
    Contains 1 GATA-type zinc finger.
    Contains 1 PHD-type zinc finger.
    Contains 1 PWWP domain.
  • Domain
    The PWWP domain is essential for targeting to pericentric heterochromatin.
  • Post-translational
    modifications
    Sumoylated; sumoylation disrupts the ability to interact with histone deacetylases (HDAC1 and HDAC2) and repress transcription.
  • Cellular localization
    Nucleus. Cytoplasm. Accumulates in the major satellite repeats at pericentric heterochromatin.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • DNA (cytosine 5) methyltransferase 3 alpha antibody
    • DNA (cytosine 5) methyltransferase 3A antibody
    • DNA (cytosine-5)-methyltransferase 3A antibody
    • DNA cytosine methyltransferase 3A2 antibody
    • DNA methyltransferase 3 alpha antibody
    • DNA methyltransferase 3a antibody
    • DNA methyltransferase HsaIIIA antibody
    • DNA MTase HsaIIIA antibody
    • DNM3A_HUMAN antibody
    • DNMT 3a antibody
    • DNMT antibody
    • Dnmt3a antibody
    • DNMT3A2 antibody
    • M.HsaIIIA antibody
    • MCMT antibody
    • OTTHUMP00000201149 antibody
    • TBRS antibody
    see all

Images

  • Western blot - Anti-Dnmt3a antibody (ab4897)
    Western blot - Anti-Dnmt3a antibody (ab4897)
    Anti-Dnmt3a antibody (ab4897) + T47-D cell lysate

    Secondary
    HRP-conjugated anti-rabbit IgG
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt3a antibody (ab4897)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt3a antibody (ab4897)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocarcinoma tissue labelling Dnmt3a with ab4897.

References

This product has been referenced in:
  • Ma M  et al. Preeclampsia is associated with hypermethylation of IGF-1 promoter mediated by DNMT1. Am J Transl Res 10:16-39 (2018). IHC-P ; Human . Read more (PubMed: 29422991) »
  • Bogoi RN  et al. Expression profiling of chromatin-modifying enzymes and global DNA methylation in CD4+ T cells from patients with chronic HIV infection at different HIV control and progression states. Clin Epigenetics 10:20 (2018). Read more (PubMed: 29449904) »

See all 5 Publications for this product

Publishing research using ab4897? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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11 reviews or Q&A

10
Votes: Highest First

Western blot abreview for Anti-Dnmt3a antibody

 Inconclusive
Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Purified protein (Brain (Cortex))
Gel Running Conditions
Reduced Denaturing (8% gel)
Loading amount
20 µg
Specification
Brain (Cortex)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Read More
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Username

Diana Christian

Verified customer

Submitted May 03 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Dnmt3a antibody

 Inconclusive
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Testis)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Testis
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde
Read More
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Username

Mr. Carl Hobbs

Verified customer

Submitted Dec 14 2015

IHC-FoFr with mouse tissue
sees cytoplasmic staining but no nuclear staining
perm:TX-100
fix: PFA 30 min
block: NGS
Ab: 1/800 - 1/50
AR: tried low heat
detection: Cy3, works well

Thank you for your phone call.
I am sorry that the antibody is not working as expected. As we discussed, I have arranged for a free of charge replacement with ab4897. Please see details below. The item is currently backordered, but the estimated de...

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I have a question regarding the cross-species reactivity of the Anti-Dnmt3a antibody (ab4897). Can you confirm whether the synthetic peptide used to raise the antibody is 100% homolog to rat and mouse? I am also interested if further experimental evide...

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Thank you very much for your interest in ab4897.

To our knowledge, ab4897 has not been tested in Mouse and Rat.It is very likely that ab4897works in these species since thehomolgy between the immunogen peptide and the proteins of interest is 1...

Read More

Dear Technical Team,
Thank you for your extensive reply - there's lots for me to try out!
Please could you tell me if the blocking peptide for ab119282 is
available to purchase from Abcam and if so could you provide me with a
produc...

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Thank you for your reply.
I am sorry to confirm it is not possible to provide a blocking peptide on this occasion. I apologise for the inconvenience, I understand this would have been useful in this case.

I hope the other suggestions pr...

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Inquiry: Antibodies ab4897 lot GR32135-2 and ab119282 lot GR75331-1 (anti Dnmt3a and anti Dnmt3b). I am looking at cytoplasmic and nuclear lysates from HT29 and C4 II cells. I have used an NK-PER kit from Pierce to prepare the lysates, protease inhibit...

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Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from these antibody.

I would like to reassure you that these antibodies are tested and covered by our 6 month guarantee fo...

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Immunoprecipitation abreview for Anti-Dnmt3a antibody

 Good
Abreviews
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (lung carcinoma)
Total protein in input
300 µg
Specification
lung carcinoma
Immuno-precipitation step
Protein G
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Username

Abcam user community

Verified customer

Submitted Jan 19 2011

Immunocytochemistry/ Immunofluorescence abreview for Anti-Dnmt3a antibody

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (hESC, mESC)
Specification
hESC, mESC
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X-100 in PBS for 15min at room temp.
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 21°C
Read More
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Username

Abcam user community

Verified customer

Submitted Sep 11 2007

What is recommended to use as a positive control for this antibody in Western blotting?

The originator used T47-D cell lysate as a positive control for ab4897 in Western blotting. If you have any more questions, please let us know.

I want to know if this Dnmt3a antibody (ab4897) could work on IHC-P Immunohistochemistry (Formalin-fixed paraffin-embedded sections)

To our knowledge, this antibody has yet to be tested in this application. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will fo...

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1-10 of 11 Abreviews or Q&A

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