Western blot abreview for Anti-Dnmt3a antibody [EPR18455]

Good
Abreviews
Application
Western blot
Sample
Astatotilapia burtoni Cell lysate - whole cell (Brain and gonads)
Gel Running Conditions
Reduced Denaturing (5% acrylamide gel)
Loading amount
25 µg
Specification
Brain and gonads
Blocking step
Milk as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Other product details

Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Skim milk
Dilution
1/1000

Secondary antibody

Dilution
1/1000
Name

Detection

Detection method
ECL
Negative control
Gonad extracts (low expression of DNMT3A transcript)
Exposure
15 hour(s), 15 minute(s) and 0 second(s)
Bands
Specific: ~110 kDa
Positive control
Brain extracts (overexpression of DNMT3A transcript)

Additional data

Additional Notes
The cross species reactivity of this antibody is awesome. However, incubation times were particularly long compared to what I would expect had we done this in a Mouse/Human sample Lane 1: protein ladder Lane 2: empty Lane 3: Brain whole cell lysate Lane 4: Gonad whole cell lysate
PubMed ID
N/A: View

Sebastian Alvarado

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Submitted Aug 07 2015

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