Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Dnmt3a antibody [EPR18455]

Good
Abreviews
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Astatotilapia burtoni Tissue sections (thalamus/hypothalamus)
Antigen retrieval step
None
Permeabilization
Yes - 0.2% TritonX
Specification
thalamus/hypothalamus
Blocking step
10% normal serum 1% BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS + 1% BSA
Dilution
1/100

Secondary antibody

Dilution
1/1000

Additional data

Additional Notes
Staining was much lower than expected. It worked but I have a feeling it would have worked better with an antigen retrieval step.
PubMed ID
N/A: View

Sebastian Alvarado

Verified customer

Submitted Dec 18 2015

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