Overview

  • Product name

    Anti-Dnmt3a antibody - N-terminal
    See all Dnmt3a primary antibodies
  • Description

    Rabbit polyclonal to Dnmt3a - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment within Human Dnmt3a (N terminal). The exact sequence is proprietary.
    Database link: Q9Y6K1

  • Positive control

    • WB: HEK-293T, Neuro-2a, PC-12 and Rat2 whole cell extracts; HEK-293T nuclear extract. IP: HEK-293T whole cell and nuclear extracts.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin
    Constituents: PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab227725 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/10000.
IP 1/100 - 1/500.

Target

  • Function

    Required for genome wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZNF238. Can actively repress transcription through the recruitment of HDAC activity.
  • Tissue specificity

    Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung.
  • Sequence similarities

    Belongs to the C5-methyltransferase family.
    Contains 1 ADD domain.
    Contains 1 GATA-type zinc finger.
    Contains 1 PHD-type zinc finger.
    Contains 1 PWWP domain.
  • Domain

    The PWWP domain is essential for targeting to pericentric heterochromatin.
  • Post-translational
    modifications

    Sumoylated; sumoylation disrupts the ability to interact with histone deacetylases (HDAC1 and HDAC2) and repress transcription.
  • Cellular localization

    Nucleus. Cytoplasm. Accumulates in the major satellite repeats at pericentric heterochromatin.
  • Information by UniProt
  • Database links

  • Alternative names

    • DNA (cytosine 5) methyltransferase 3 alpha antibody
    • DNA (cytosine 5) methyltransferase 3A antibody
    • DNA (cytosine-5)-methyltransferase 3A antibody
    • DNA cytosine methyltransferase 3A2 antibody
    • DNA methyltransferase 3 alpha antibody
    • DNA methyltransferase 3a antibody
    • DNA methyltransferase HsaIIIA antibody
    • DNA MTase HsaIIIA antibody
    • DNM3A_HUMAN antibody
    • DNMT 3a antibody
    • DNMT antibody
    • Dnmt3a antibody
    • DNMT3A2 antibody
    • M.HsaIIIA antibody
    • MCMT antibody
    • OTTHUMP00000201149 antibody
    • TBRS antibody
    see all

Images

  • All lanes : Anti-Dnmt3a antibody - N-terminal (ab227725) at 1/3000 dilution

    Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lanes 2-3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with Dnmt3a shRNA, whole cell extract

    Lysates/proteins at 30 µg per lane.


    5% SDS-PAGE gel.

  • All lanes : Anti-Dnmt3a antibody - N-terminal (ab227725) at 1/5000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) nuclear extract

    Lysates/proteins at 30 µg per lane.


    5% SDS-PAGE gel.

  • Dnmt3a was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) nuclear extract with ab227725. Western blot was performed from the immunoprecipitate using ab227725 at 1/2000 dilution. Anti-rabbit IgG was used as secondary antibody.

    Lane 1: HEK-293T nuclear extract 40 µg (Input). 

    Lane 2: 4 µg preimmune rabbit IgG instead of ab227725 in HEK-293T nuclear extract.

    Lane 3: ab227725 IP in HEK-293T nuclear extract. 

  • Dnmt3a was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with ab227725. Western blot was performed from the immunoprecipitate using ab227725. Anti-rabbit IgG was used as secondary antibody.

    Lane 1: HEK-293T whole cell exract (Input). 

    Lane 2: Control IgG instead of ab227725 in HEK-293T whole cell extract.

    Lane 3: ab227725 IP in HEK-293T whole cell extract. 

  • Anti-Dnmt3a antibody - N-terminal (ab227725) at 1/1000 dilution + Neuro-2a (mouse neuroblastoma cell line) whole cell extract at 30 µg

    Secondary
    HRP-conjugated anti-rabbit IgG


    7.5% SDS-PAGE gel.

  • All lanes : Anti-Dnmt3a antibody - N-terminal (ab227725) at 1/1000 dilution

    Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract
    Lane 2 : Rat2 (rat fibroblast cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG


    7.5% SDS-PAGE gel.

References

ab227725 has not yet been referenced specifically in any publications.

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