Key features and details
- Rabbit polyclonal to Dnmt3a (phospho S390 + S393)
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Dnmt3a (phospho S390 + S393) antibody
See all Dnmt3a primary antibodies
DescriptionRabbit polyclonal to Dnmt3a (phospho S390 + S393)
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Rat, Sheep, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan
- This antibody gave a positive signal in HEK293 whole cell lysate. This antibody gave a positive signal in the following methanol fixed cell lines: HepG2.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab87763 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 102 kDa).|
FunctionRequired for genome wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZNF238. Can actively repress transcription through the recruitment of HDAC activity.
Tissue specificityHighly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung.
Sequence similaritiesBelongs to the C5-methyltransferase family.
Contains 1 ADD domain.
Contains 1 GATA-type zinc finger.
Contains 1 PHD-type zinc finger.
Contains 1 PWWP domain.
DomainThe PWWP domain is essential for targeting to pericentric heterochromatin.
modificationsSumoylated; sumoylation disrupts the ability to interact with histone deacetylases (HDAC1 and HDAC2) and repress transcription.
Cellular localizationNucleus. Cytoplasm. Accumulates in the major satellite repeats at pericentric heterochromatin.
- Information by UniProt
- DNA (cytosine 5) methyltransferase 3 alpha antibody
- DNA (cytosine 5) methyltransferase 3A antibody
- DNA (cytosine-5)-methyltransferase 3A antibody
All lanes : Anti-Dnmt3a (phospho S390 + S393) antibody (ab87763) at 1 µg/ml
Lane 1 : HEK293 (Human) Whole Cell Lysate (ab52256)
Lane 2 : HEK293 (Human) Whole Cell Lysate (ab52256) with Phospho S390 + S393 peptide at 1 µg/ml
Lane 3 : HEK293 (Human) Whole Cell Lysate (ab52256) with Phospho S390 peptide at 1 µg/ml
Lane 4 : HEK293 (Human) Whole Cell Lysate (ab52256) with Phospho S393 peptide at 1 µg/ml
Lane 5 : HEK293 (Human) Whole Cell Lysate (ab52256) with Non modified control peptide at 1 µg/ml
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 102 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Additional bands at: 39 kDa, 52 kDa, 68 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Lysates from U2OS WT cells were loaded as input. The western blot was probed with anti Dnmt3a (phospho S390 + S393) antibody (ab87763) either in the presence or absence of double modified peptide phosphorylated on S390 and S393 as indicated in the figure. Detected phospho Dnmt3a is indicated by the arrow. Please note: the observed band migrates at a higher MW than predicted probably because of phosphorylation. This data was provided by an anonymous collaborator.
ICC/IF image of ab87763 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab87763 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab87763 has been referenced in 2 publications.
- Fan L et al. Enhancement of the chondrogenic differentiation of mesenchymal stem cells and cartilage repair by ghrelin. J Orthop Res N/A:N/A (2019). PubMed: 30644571
- Deplus R et al. Regulation of DNA methylation patterns by CK2-mediated phosphorylation of Dnmt3a. Cell Rep 8:743-53 (2014). WB ; Mouse, Human . PubMed: 25066127