Overview

  • Product name

    Anti-Dnmt3b antibody [4D1A2E5]
    See all Dnmt3b primary antibodies
  • Description

    Mouse monoclonal [4D1A2E5] to Dnmt3b
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human Dnmt3b aa 1-150. Expressed in E.coli.
    Database link: Q9UBC3

  • Positive control

    • WB: Human Dnmt3b (AA: 1-150) recombinant protein; Dnmt3b (AA: 1-150)-hIgGFc transfected HEK-293 cell lysate. ICC/IF: HeLa cells. Flow: K562 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    4D1A2E5
  • Isotype

    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab233798 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500.
Flow Cyt 1/200 - 1/400.
ICC/IF 1/200 - 1/1000.

Target

  • Function

    Required for genome wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing (By similarity). In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Isoforms 4 and 5 are probably not functional due to the deletion of two conserved methyltransferase motifs.
  • Tissue specificity

    Ubiquitous; highly expressed in fetal liver, heart, kidney, placenta, and at lower levels in spleen, colon, brain, liver, small intestine, lung, peripheral blood mononuclear cells, and skeletal muscle. Isoform 1 is expressed in all tissues except brain, skeletal muscle and PBMC, 3 is ubiquitous, 4 is expressed in all tissues except brain, skeletal muscle, lung and prostate and 5 is detectable only in testis and at very low level in brain and prostate.
  • Involvement in disease

    Defects in DNMT3B are a cause of immunodeficiency-centromeric instability-facial anomalies syndrome (ICF) [MIM:242860]. ICF is a rare autosomal recessive disorder characterized by a variable immunodeficiency, mild facial anomalies, and centromeric heterochromatin instability involving chromosomes 1, 9, and 16. ICF is biochemically characterized by hypomethylation of CpG sites in some regions of heterochromatin.
  • Sequence similarities

    Belongs to the C5-methyltransferase family.
    Contains 1 ADD domain.
    Contains 1 GATA-type zinc finger.
    Contains 1 PHD-type zinc finger.
    Contains 1 PWWP domain.
  • Domain

    The PWWP domain is essential for targeting to pericentric heterochromatin.
  • Post-translational
    modifications

    Sumoylated.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cytosine 5methyltransferase 3B antibody
    • DNA antibody
    • DNA (cytosine 5) methyltransferase 3 beta antibody
    • DNA (cytosine 5)-methyltransferase 3B antibody
    • DNA (cytosine-5)-methyltransferase 3B antibody
    • DNA methyltransferase HsaIIIB antibody
    • DNA MTase HsaIIIB antibody
    • DNM3B_HUMAN antibody
    • Dnmt3b antibody
    • EC 2.1.1.37 antibody
    • ICF antibody
    • ICF1 antibody
    • M.HsaIIIB antibody
    • MGC124407 antibody
    • RP23-89H14.3 antibody
    see all

Images

  • Anti-Dnmt3b antibody [4D1A2E5] (ab233798) at 1/100 dilution + Human Dnmt3b (AA: 1-150) recombinant protein.


    Expected MW is 42.6 kDa.

  • All lanes : Anti-Dnmt3b antibody [4D1A2E5] (ab233798) at 1/100 dilution

    Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate.
    Lane 2 : Dnmt3b (AA: 1-150)-hIgGFc transfected HEK-293 cell lysate.
  • Immunofluorescent analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Dnmt3b using ab233798 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

  • Flow cytometric analysis of K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling Dnmt3b using ab233798 at 1/200 dilution (green) and the negative control (red).

References

ab233798 has not yet been referenced specifically in any publications.

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