Key features and details
- Rabbit polyclonal to Dnmt3b
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Dnmt3b antibody
See all Dnmt3b primary antibodies
DescriptionRabbit polyclonal to Dnmt3b
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide derived from the N terminal region of Human Dnmt3b, conjugated to KLH
- Human testis tissue, HT29 cell lysate, HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 48% PBS, 0.88% Sodium chloride, 50% Glycerol
PBS is without Mg2+, Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab119282 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 96 kDa.|
|IHC-P||Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/500.|
FunctionRequired for genome wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing (By similarity). In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Isoforms 4 and 5 are probably not functional due to the deletion of two conserved methyltransferase motifs.
Tissue specificityUbiquitous; highly expressed in fetal liver, heart, kidney, placenta, and at lower levels in spleen, colon, brain, liver, small intestine, lung, peripheral blood mononuclear cells, and skeletal muscle. Isoform 1 is expressed in all tissues except brain, skeletal muscle and PBMC, 3 is ubiquitous, 4 is expressed in all tissues except brain, skeletal muscle, lung and prostate and 5 is detectable only in testis and at very low level in brain and prostate.
Involvement in diseaseDefects in DNMT3B are a cause of immunodeficiency-centromeric instability-facial anomalies syndrome (ICF) [MIM:242860]. ICF is a rare autosomal recessive disorder characterized by a variable immunodeficiency, mild facial anomalies, and centromeric heterochromatin instability involving chromosomes 1, 9, and 16. ICF is biochemically characterized by hypomethylation of CpG sites in some regions of heterochromatin.
Sequence similaritiesBelongs to the C5-methyltransferase family.
Contains 1 ADD domain.
Contains 1 GATA-type zinc finger.
Contains 1 PHD-type zinc finger.
Contains 1 PWWP domain.
DomainThe PWWP domain is essential for targeting to pericentric heterochromatin.
- Information by UniProt
- Cytosine 5methyltransferase 3B antibody
- DNA antibody
- DNA (cytosine 5) methyltransferase 3 beta antibody
All lanes : Anti-Dnmt3b antibody (ab119282) at 1/500 dilution
Lane 1 : HT-29 cell lysate
Lane 2 : HT-29 cell lysate with blocking peptide
Predicted band size: 96 kDa
ab119282, at 10 µg/ml, staining Dnmt3b in Human testis tissue by immunohistochemistry.
ab119282, at 1/100, staining Dnmt3b in HeLa cells by immunofluorescence. The image on the right shows cells treated with blocking peptide.
ab119282 has been referenced in 3 publications.
- Akiyama N et al. Regulation of DNA methylation levels in the process of oral mucosal regeneration in a rat oral ulcer model. Histol Histopathol N/A:18147 (2019). PubMed: 31286466
- Ma M et al. Preeclampsia is associated with hypermethylation of IGF-1 promoter mediated by DNMT1. Am J Transl Res 10:16-39 (2018). IHC-P ; Human . PubMed: 29422991
- Gailhouste L et al. Differentiation Therapy by Epigenetic Reconditioning Exerts Antitumor Effects on Liver Cancer Cells. Mol Ther N/A:N/A (2018). WB . PubMed: 29759938