Overview

  • Product name

  • Description

    Rabbit polyclonal to Dnmt3b
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Fr, WB, IP, ICC/IF, ChIPmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Recombinant fragment, corresponding to amino acids 1-181 of Mouse Dnmt3b (AF068626)

  • Positive control

    • Solubilized mouse embryos at the stages of E7.0-E16.5; transiently transfected 293T cells; E4.5, 5.5 and 7.0 mouse embryo sections.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, Ammonium sulphate

    trace of ammonium sulphate only
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122932 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/2000 - 1/5000.
WB 1/3000. Predicted molecular weight: 96 kDa.
IP 1/1000.
ICC/IF 1/5000.
ChIP 1/1000.

Target

  • Function

    Required for genome wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing (By similarity). In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Isoforms 4 and 5 are probably not functional due to the deletion of two conserved methyltransferase motifs.
  • Tissue specificity

    Ubiquitous; highly expressed in fetal liver, heart, kidney, placenta, and at lower levels in spleen, colon, brain, liver, small intestine, lung, peripheral blood mononuclear cells, and skeletal muscle. Isoform 1 is expressed in all tissues except brain, skeletal muscle and PBMC, 3 is ubiquitous, 4 is expressed in all tissues except brain, skeletal muscle, lung and prostate and 5 is detectable only in testis and at very low level in brain and prostate.
  • Involvement in disease

    Defects in DNMT3B are a cause of immunodeficiency-centromeric instability-facial anomalies syndrome (ICF) [MIM:242860]. ICF is a rare autosomal recessive disorder characterized by a variable immunodeficiency, mild facial anomalies, and centromeric heterochromatin instability involving chromosomes 1, 9, and 16. ICF is biochemically characterized by hypomethylation of CpG sites in some regions of heterochromatin.
  • Sequence similarities

    Belongs to the C5-methyltransferase family.
    Contains 1 ADD domain.
    Contains 1 GATA-type zinc finger.
    Contains 1 PHD-type zinc finger.
    Contains 1 PWWP domain.
  • Domain

    The PWWP domain is essential for targeting to pericentric heterochromatin.
  • Post-translational
    modifications

    Sumoylated.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cytosine 5methyltransferase 3B antibody
    • DNA antibody
    • DNA (cytosine 5) methyltransferase 3 beta antibody
    • DNA (cytosine 5)-methyltransferase 3B antibody
    • DNA (cytosine-5)-methyltransferase 3B antibody
    • DNA methyltransferase HsaIIIB antibody
    • DNA MTase HsaIIIB antibody
    • DNM3B_HUMAN antibody
    • Dnmt3b antibody
    • EC 2.1.1.37 antibody
    • ICF antibody
    • ICF1 antibody
    • M.HsaIIIB antibody
    • MGC124407 antibody
    • RP23-89H14.3 antibody
    see all

Images

  • All lanes : Anti-Dnmt3b antibody (ab122932) at 1/3000 dilution

    Lane 1 : wild-type mouse embryonic stem cell lysate with 5% skim milk
    Lane 2 : Dnmt3b/3a knockout mouse embryonic stem cell lysate with 5% skim milk

    Predicted band size: 96 kDa

  • Immunocytochemistry/ Immunofluorescence analysis of wild-type (left) and Dnmt3b/3a knockout (right) mouse embryonic stem cells using ab122932 at 1/5000 dilution. DNA was stained with DAPI (bottom). Cell were fixed with 4% PFA/PBS for 30 min at 4℃. 

  • All lanes : Anti-Dnmt3b antibody (ab122932) at 1/1000 dilution

    Lane 1 : stages E7.0 solubilized mouse embryo at 10 µg
    Lane 2 : stages E8.5 solubilized mouse embryo at 10 µg
    Lane 3 : stages E9.5 solubilized mouse embryo at 10 µg
    Lane 4 : stages E10.5 solubilized mouse embryo at 10 µg
    Lane 5 : stages E12.5 solubilized mouse embryo at 10 µg
    Lane 6 : stages E14.5 solubilized mouse embryo at 10 µg
    Lane 7 : stages E16.5 solubilized mouse embryo at 10 µg
    Lane 8 : 293T cells transiently transfected with 3b1 isoform of Dnmt3b
    Lane 9 : 293T cells transiently transfected with 3b2 isoform of Dnmt3b
    Lane 10 : 293T cells transiently transfected with 3b3 isoform of Dnmt3b

    Predicted band size: 96 kDa



    The embryos were solubilized by sonication or homogenization in the presence of 0.1% SDS.
  • Expression of Dnmt3b in mouse embyos was examined by immuno-fluorescence staining using 1/1000 ab122932.
    Mouse embryos at E4.5, 5.5 and 7.0 were fixed in cold acetone and stained with ab122932 or DAPI (blue). The antibody was detected with anti-rabbit IgG conjugated with ALEXA568 (red). The ICM (the inner cell mass) and trophectoderm (T) are indicated by arrows and arrowheads, respectively. The epiblast (E), and the embryonic ectoderm (EE) and extraembryonic region (EX) are indicated by square brackets. Dnmt3b existed at E4.5-7.0, in ICM at E4.5, the epiblast at E5.5, and the embryonic ectoderm at E7.0.

References

This product has been referenced in:

  • Casey AE  et al. Mammary molecular portraits reveal lineage-specific features and progenitor cell vulnerabilities. J Cell Biol 217:2951-2974 (2018). Read more (PubMed: 29921600) »
  • Poole CJ  et al. DNMT3B overexpression contributes to aberrant DNA methylation and MYC-driven tumor maintenance in T-ALL and Burkitt's lymphoma. Oncotarget 8:76898-76920 (2017). Read more (PubMed: 29100357) »
See all 5 Publications for this product

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