Key features and details
- Rabbit polyclonal to Dnmt3b
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Dnmt3b antibody
See all Dnmt3b primary antibodies
DescriptionRabbit polyclonal to Dnmt3b
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Mouse Dnmt3b conjugated to keyhole limpet haemocyanin. 3 KLH-conjugated synthetic peptides containing sequences from different parts of the protein.
Database link: O88509
- WB: HeLa whole cell extracts. ICC/IF: HeLa cells.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservatives: 0.05% Sodium azide, 0.05% Proclin 300
Concentration information loading...
Our Abpromise guarantee covers the use of ab232834 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FunctionRequired for genome wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing (By similarity). In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Isoforms 4 and 5 are probably not functional due to the deletion of two conserved methyltransferase motifs.
Tissue specificityUbiquitous; highly expressed in fetal liver, heart, kidney, placenta, and at lower levels in spleen, colon, brain, liver, small intestine, lung, peripheral blood mononuclear cells, and skeletal muscle. Isoform 1 is expressed in all tissues except brain, skeletal muscle and PBMC, 3 is ubiquitous, 4 is expressed in all tissues except brain, skeletal muscle, lung and prostate and 5 is detectable only in testis and at very low level in brain and prostate.
Involvement in diseaseDefects in DNMT3B are a cause of immunodeficiency-centromeric instability-facial anomalies syndrome (ICF) [MIM:242860]. ICF is a rare autosomal recessive disorder characterized by a variable immunodeficiency, mild facial anomalies, and centromeric heterochromatin instability involving chromosomes 1, 9, and 16. ICF is biochemically characterized by hypomethylation of CpG sites in some regions of heterochromatin.
Sequence similaritiesBelongs to the C5-methyltransferase family.
Contains 1 ADD domain.
Contains 1 GATA-type zinc finger.
Contains 1 PHD-type zinc finger.
Contains 1 PWWP domain.
DomainThe PWWP domain is essential for targeting to pericentric heterochromatin.
- Information by UniProt
- Cytosine 5methyltransferase 3B antibody
- DNA antibody
- DNA (cytosine 5) methyltransferase 3 beta antibody
Anti-Dnmt3b antibody (ab232834) at 1/1000 dilution + HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell extracts at 25 µg
Dilution buffer: TBS-Tween containing 5% skimmed milk.
HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for Dnmt3b using ab232834 at a dilution of 1/1000 in ICC/IF, followed by an anti-rabbit antibody conjugated to Alexa-Fluor®488 (Left panel, green).
Cells were fxed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The right panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the bottom panel.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab232834 has not yet been referenced specifically in any publications.