Overview

  • Product name

    Anti-Dnmt3b antibody - N-terminal
    See all Dnmt3b primary antibodies
  • Description

    Rabbit polyclonal to Dnmt3b - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Dnmt3b (N terminal). The exact sequence is proprietary.
    Database link: Q9UBC3

  • Positive control

    • WB: WT HeLa cell extract; HeLa whole cell and nuclear extracts. IHC-P: Human lung carcinoma tissue. ICC/IF: HeLa cells. IP: K562 nuclear extract.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin
    Constituents: PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab227883 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 96 kDa.
IP 1/100 - 1/500.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Required for genome wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing (By similarity). In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Isoforms 4 and 5 are probably not functional due to the deletion of two conserved methyltransferase motifs.
  • Tissue specificity

    Ubiquitous; highly expressed in fetal liver, heart, kidney, placenta, and at lower levels in spleen, colon, brain, liver, small intestine, lung, peripheral blood mononuclear cells, and skeletal muscle. Isoform 1 is expressed in all tissues except brain, skeletal muscle and PBMC, 3 is ubiquitous, 4 is expressed in all tissues except brain, skeletal muscle, lung and prostate and 5 is detectable only in testis and at very low level in brain and prostate.
  • Involvement in disease

    Defects in DNMT3B are a cause of immunodeficiency-centromeric instability-facial anomalies syndrome (ICF) [MIM:242860]. ICF is a rare autosomal recessive disorder characterized by a variable immunodeficiency, mild facial anomalies, and centromeric heterochromatin instability involving chromosomes 1, 9, and 16. ICF is biochemically characterized by hypomethylation of CpG sites in some regions of heterochromatin.
  • Sequence similarities

    Belongs to the C5-methyltransferase family.
    Contains 1 ADD domain.
    Contains 1 GATA-type zinc finger.
    Contains 1 PHD-type zinc finger.
    Contains 1 PWWP domain.
  • Domain

    The PWWP domain is essential for targeting to pericentric heterochromatin.
  • Post-translational
    modifications

    Sumoylated.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cytosine 5methyltransferase 3B antibody
    • DNA antibody
    • DNA (cytosine 5) methyltransferase 3 beta antibody
    • DNA (cytosine 5)-methyltransferase 3B antibody
    • DNA (cytosine-5)-methyltransferase 3B antibody
    • DNA methyltransferase HsaIIIB antibody
    • DNA MTase HsaIIIB antibody
    • DNM3B_HUMAN antibody
    • Dnmt3b antibody
    • EC 2.1.1.37 antibody
    • ICF antibody
    • ICF1 antibody
    • M.HsaIIIB antibody
    • MGC124407 antibody
    • RP23-89H14.3 antibody
    see all

Images

  • All lanes : Anti-Dnmt3b antibody - N-terminal (ab227883) at 1/500 dilution

    Lane 1 : WT HeLa cell extract
    Lane 2 : Dnmt3b Knockout HeLa cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 96 kDa

  • All lanes : Anti-Dnmt3b antibody - N-terminal (ab227883) at 1/500 dilution

    Lane 1 : Wild-type HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
    Lane 2 : Dnmt3b knockout HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 96 kDa



    7.5% SDS-PAGE gel.

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Dnmt3b (green) using ab227883 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.

    Red: phalloidin, a cytoskeleton marker, at 1/50 dilution.

  • Paraffin-embedded human lung carcinoma tissue stained for Dnmt3b using ab227883 at 1/250 dilution in immunohistochemical analysis.

  • All lanes : Anti-Dnmt3b antibody - N-terminal (ab227883) at 1/500 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) nuclear extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 96 kDa



    5% SDS-PAGE gel.

  • Dnmt3b was immunoprecipitated from K562 (human chronic myelogenous leukemia cell line from bone marrow ) nuclear extract with 5 µg ab227883. Western blot was performed from the immunoprecipitate using ab227883. Anti-Rabbit IgG was used as a secondary reagent.

    Lane 1: Control IgG IP in K562 nuclear extract.

    Lane 2: ab227883 IP in K562 nuclear extract.

References

ab227883 has not yet been referenced specifically in any publications.

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