- Datasheet
- Specific References (7)
- Protocols
Overview
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Product nameAnti-Dnmt3L antibody
See all Dnmt3L primary antibodies -
DescriptionRabbit polyclonal to Dnmt3L
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Host speciesRabbit
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SpecificityThis antibody is expected to cross-react with mouse as the sequence of the human immunogen shows 76% identity with mouse.
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Tested applicationsSuitable for: ICC/IF, ELISA, WB, IPmore details
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Species reactivityReacts with: Mouse, Rat, Hamster, Cow, Human
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Immunogen
Synthetic peptide corresponding to Human Dnmt3L aa 152-164 conjugated to keyhole limpet haemocyanin.
Sequence:C-GLLQRRRKWRSQL
Database link: Q9UJW3 -
General notes
DNMT 3L is a nuclear protein which has similarity to DNA methyltransferases, involved in de novo methylation of CpG islands. CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases. This protein is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, this protein does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and it is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2 -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesThis is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. This antibody is expected to cross-react with mouse as the sequence of the human immunogen shows 76% identity with mouse. Reactivity with DNMT3L from other species has not yet been tested.
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Primary antibody notesDNMT 3L is a nuclear protein which has similarity to DNA methyltransferases, involved in de novo methylation of CpG islands. CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases. This protein is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, this protein does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and it is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab3493 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/500. | |
| ELISA | 1/1000 - 1/3000. 1/1000 - 1/3000 against 0.1µg of the immunizing peptide. | |
| WB | Use a concentration of 0.4 µg/ml. Predicted molecular weight: 43.6 kDa. Use at an assay dependent dilution. The target may need to be immunoprecipitated to identify the target in WB. Predicted molecular weight: 43.6 kDa. | |
| IP | 1/100. |
Target
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FunctionCatalytically inactive regulatory factor of DNA methyltransferases. It is essential for the function of DNMT3A and DNMT3B. Activates DNMT3A and DNMT3B by binding to their catalytic domain. Accelerates the binding of DNA and AdoMet to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases. Recognizes unmethylated histone H3 lysine 4 (H3K4) and induces de novo DNA methylation by recruitment or activation of DNMT3.
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Tissue specificityExpressed at low levels in several tissues including testis, ovary, and thymus.
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Sequence similaritiesBelongs to the C5-methyltransferase family.
Contains 1 ADD-type zinc finger. -
Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 613785 Cow
- Entrez Gene: 29947 Human
- Entrez Gene: 54427 Mouse
- Entrez Gene: 309680 Rat
- Omim: 606588 Human
- SwissProt: Q9UJW3 Human
- SwissProt: Q9CWR8 Mouse
- SwissProt: Q1LZ50 Rat
see all -
Alternative names
- Cytosine 5 methyltransferase 3 like protein antibody
- DNA (cytosine 5 ) methyltransferase 3 like antibody
- DNA (cytosine-5)-methyltransferase 3-like antibody
see all
Images
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Immunoprecipitation - Anti-Dnmt3L antibody (ab3493)Transfection of U2OS cells (10cm dish) using 3
µ g of GAL4-DNMT3L. Protein extraction using IPH 150mM. IP and WB conditions, see Fuks et al. (2000). Nature Genetics 24: 88-91.WB using 0.4µ g/ml in TBS milk 2%, BSA 0.5%. Dectection using ECL.Note: ab3493 worked in WB following IP, but did not work in a straight WB.
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Western blot - Anti-Dnmt3L antibody (ab3493)This image is courtesy of an anonymous AbreviewAnti-Dnmt3L antibody (ab3493) at 1/1000 dilution + Rat C6 cells, whole cell lysate at 25 µg
Secondary
HRP conjugated goat anti-rabbit antibody at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 43.6 kDa
Observed band size: 52 kDa (why is the actual band size different from the predicted?)
Exposure time: 30 seconds
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Immunocytochemistry/ Immunofluorescence - Anti-Dnmt3L antibody (ab3493)Image from Zuccotti M et al., BMC Genomics. 2011 Jul 5;12:1-13. doi: 10.1186/1471-2164-12-345.; Fig 3.; 5 July 2011, BMC Genomics 2011, 12:345Immunofluorescence analysis of 2-cell mouse embryos, staining Dnmt3L with ab3493.
Embryos were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 0.5% blocking reagent in TNT (0.1 M, Tris-HCl, pH 7.5, 0.15 M NaCl, 0.05% Tween-20) buffer for 20 min at 4°C. Embryos were incaubted with primary antibody (1/500) for 1 hour at 37°C. An AlexaFluor®488-conjugated goat anti-rabbit IgG (1/400) was used as the secondary antibody.
References
This product has been referenced in:
- Nanan KK et al. Independence between pre-mRNA splicing and DNA methylation in an isogenic minigene resource. Nucleic Acids Res 45:12780-12797 (2017). Read more (PubMed: 29244186) »
- Lee SM et al. HBx induces hypomethylation of distal intragenic CpG islands required for active expression of developmental regulators. Proc Natl Acad Sci U S A 111:9555-60 (2014). Mouse . Read more (PubMed: 24941955) »
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