Western blot abreview for Anti-Dnmt3L antibody

Excellent
Abreviews
Application
Western blot
Sample
Rat Cell lysate - whole cell (C6 Cell line)
Loading amount
25 µg
Specification
C6 Cell line
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C

Secondary antibody

Name
Non-Abcam antibody was used: anti-rabbit antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/3000

Detection

Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 52 kDa

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Submitted Jun 04 2008

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