Western blot abreview for Anti-Dnmt3L antibody

Excellent
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (NIH 3T3)
Loading amount
30 µg
Specification
NIH 3T3
Gel Running Conditions
Reduced Denaturing (8% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/1000
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2500

Detection

Detection method
ECL
Exposure
2 second(s)
Bands
Specific: 36 kDa

Additional data

Additional Notes
The reasons I got 36 kD band instead of predicted 47 kD band are first the sequence of mouse Dnmt3L is different than that of human, second, I used 8% gel to run the sample, the marker might not be very accurate in the low concentration gel. I should have ran the sample in higher concentraion gel, however I had other samples whose molecular weights are much higher (around 300 kD).

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Submitted Jun 10 2008

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