The reasons I got 36 kD band instead of predicted 47 kD band are first the sequence of mouse Dnmt3L is different than that of human, second, I used 8% gel to run the sample, the marker might not be very accurate in the low concentration gel. I should have ran the sample in higher concentraion gel, however I had other samples whose molecular weights are much higher (around 300 kD).
Abcam user community
Submitted Jun 10 2008
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