Western blot abreview for Anti-Dnmt3L antibody

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - nuclear (lymphoma cell line)
Loading amount
150 µg
Specification
lymphoma cell line
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 21°C

Other product details

Dilution
1/200
Incubation time
20 hour(s) and 30 minute(s) · Temperature: 4°C · Diluent: 5% non fat milk in TBST

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti Rabbit IgG HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL+
Exposure
2 minute(s) and 30 second(s)
Bands
Specific: 50 kDa

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Submitted Feb 24 2010

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