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    dnmt3l-antibody-n1179-ab93613.pdf

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Epigenetics and Nuclear Signaling DNA methylation DNMTs
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Anti-Dnmt3L antibody [N117/9] (ab93613)

  • Datasheet
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Western blot - Anti-Dnmt3L antibody [N117/9] (ab93613)
  • Immunohistochemistry (Frozen sections) - Anti-Dnmt3L antibody [N117/9] (ab93613)
  • Immunocytochemistry/ Immunofluorescence - Anti-Dnmt3L antibody [N117/9] (ab93613)

Key features and details

  • Mouse monoclonal [N117/9] to Dnmt3L
  • Suitable for: WB, ICC/IF, IHC-Fr
  • Reacts with: Human
  • Isotype: IgG1

You may also be interested in

Protein
Product image
Recombinant human Dnmt3L protein (ab170406)
Secondary
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

View more associated products

Overview

  • Product name

    Anti-Dnmt3L antibody [N117/9]
    See all Dnmt3L primary antibodies
  • Description

    Mouse monoclonal [N117/9] to Dnmt3L
  • Host species

    Mouse
  • Specificity

    Detects Dnmt3L, it does not react with DNMT3a or DNMT3B.
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Frmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Fusion protein, corresponding to amino acids 1-387 of Human Dnmt3L (accession number BAA95556)

  • General notes

    The clone number has been updated from S117-9 to N117/9, both clone numbers name the same antibody clone.

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    N117/9
  • Isotype

    IgG1
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA methylation
    • DNMTs

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant human Dnmt3L protein (ab170406)

Applications

Our Abpromise guarantee covers the use of ab93613 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
IHC-Fr
  • Application notes
    ICC/IF: Use at a concentration of 1 - 10 µg/ml.
    IHC-P: Use at a concentration of 0.1 - 1 µg/ml.
    IHC-Fr: Use at a concentration of 0.1 - 1 µg/ml.
    WB: Use at a concentration of 1 - 10 µg/ml. Predicted molecular weight: 44 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Catalytically inactive regulatory factor of DNA methyltransferases. It is essential for the function of DNMT3A and DNMT3B. Activates DNMT3A and DNMT3B by binding to their catalytic domain. Accelerates the binding of DNA and AdoMet to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases. Recognizes unmethylated histone H3 lysine 4 (H3K4) and induces de novo DNA methylation by recruitment or activation of DNMT3.
    • Tissue specificity

      Expressed at low levels in several tissues including testis, ovary, and thymus.
    • Sequence similarities

      Belongs to the C5-methyltransferase family.
      Contains 1 ADD-type zinc finger.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession Q9UJW3 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 29947 Human
      • Omim: 606588 Human
      • SwissProt: Q9UJW3 Human
      • Unigene: 592165 Human
      • Alternative names

        • Cytosine 5 methyltransferase 3 like protein antibody
        • DNA (cytosine 5 ) methyltransferase 3 like antibody
        • DNA (cytosine-5)-methyltransferase 3-like antibody
        • DNA cytosine 5 methyltransferase 3 like protein antibody
        • DNA methyltransferase 3 like protein antibody
        • DNM3L_HUMAN antibody
        • Dnmt 3L antibody
        • Dnmt3l antibody
        • Human cytosine 5 methyltransferase 3 like protein antibody
        • MGC1090 antibody
        see all

      Images

      • Western blot - Anti-Dnmt3L antibody [N117/9] (ab93613)
        Western blot - Anti-Dnmt3L antibody [N117/9] (ab93613)
        Western blot detection of DNMT3L in a Human tissue mix using ab93613 at 1:1000 dilution.
      • Immunohistochemistry (Frozen sections) - Anti-Dnmt3L antibody [N117/9] (ab93613)
        Immunohistochemistry (Frozen sections) - Anti-Dnmt3L antibody [N117/9] (ab93613)

        Immunohistochemical analysis of paraffin-embedded Mouse backskin labeling Dnmt3L with ab93613 at 1 ug/ml

      • Immunocytochemistry/ Immunofluorescence - Anti-Dnmt3L antibody [N117/9] (ab93613)
        Immunocytochemistry/ Immunofluorescence - Anti-Dnmt3L antibody [N117/9] (ab93613)

        Immunofluorescent analysis on Human HaCaT cells labeling Dnmt3L with ab93613 at 1/1000 dilution.

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab93613? Please let us know so that we can cite the reference in this datasheet.

      ab93613 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Dnmt3L antibody [S117-9]

      Poor
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Blocking step
      Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
      Sample
      Marmoset (common) Tissue sections (adult testis)
      Specification
      adult testis
      Permeabilization
      No
      Fixative
      Formaldehyde
      Read More

      Dr. 煜欽 林

      Verified customer

      Submitted Jul 17 2013

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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