Anti-Dnmt3L antibody [N117/9] (ab93613)
![Western blot - Anti-Dnmt3L antibody [N117/9] (ab93613)](https://www.abcam.com/ps/products/93/ab93613/Images/ab93613-199671-anti-dnmt3l-antibody-n117-9-western-blot.jpg "Western blot - Anti-Dnmt3L antibody [N117/9] (ab93613)")
Key features and details
- Mouse monoclonal [N117/9] to Dnmt3L
- Suitable for: WB, ICC/IF, IHC-Fr
- Reacts with: Human
- Isotype: IgG1
Overview
-
Product name
Anti-Dnmt3L antibody [N117/9]
See all Dnmt3L primary antibodies -
Description
Mouse monoclonal [N117/9] to Dnmt3L -
Host species
Mouse -
Specificity
Detects Dnmt3L, it does not react with DNMT3a or DNMT3B. -
Tested applications
Suitable for: WB, ICC/IF, IHC-Frmore details -
Species reactivity
Reacts with: Human -
Immunogen
Fusion protein, corresponding to amino acids 1-387 of Human Dnmt3L (accession number BAA95556)
-
General notes
The clone number has been updated from S117-9 to N117/9, both clone numbers name the same antibody clone.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
N117/9 -
Isotype
IgG1 -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab93613 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | ||
| ICC/IF | ||
| IHC-Fr |
IHC-P: Use at a concentration of 0.1 - 1 µg/ml.
IHC-Fr: Use at a concentration of 0.1 - 1 µg/ml.
WB: Use at a concentration of 1 - 10 µg/ml. Predicted molecular weight: 44 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
-
Function
Catalytically inactive regulatory factor of DNA methyltransferases. It is essential for the function of DNMT3A and DNMT3B. Activates DNMT3A and DNMT3B by binding to their catalytic domain. Accelerates the binding of DNA and AdoMet to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases. Recognizes unmethylated histone H3 lysine 4 (H3K4) and induces de novo DNA methylation by recruitment or activation of DNMT3. -
Tissue specificity
Expressed at low levels in several tissues including testis, ovary, and thymus. -
Sequence similarities
Belongs to the C5-methyltransferase family.
Contains 1 ADD-type zinc finger. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 29947 Human
- Omim: 606588 Human
- SwissProt: Q9UJW3 Human
- Unigene: 592165 Human
-
Alternative names
- Cytosine 5 methyltransferase 3 like protein antibody
- DNA (cytosine 5 ) methyltransferase 3 like antibody
- DNA (cytosine-5)-methyltransferase 3-like antibody
see all
Images
-
Western blot - Anti-Dnmt3L antibody [N117/9] (ab93613)Western blot detection of DNMT3L in a Human tissue mix using ab93613 at 1:1000 dilution.
-
![Immunohistochemistry (Frozen sections) - Anti-Dnmt3L antibody [N117/9] (ab93613)](https://www.abcam.com/ps/products/93/ab93613/Images/ab93613-260419-anti-dnmt3l-antibody-n117-9-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-Dnmt3L antibody [N117/9] (ab93613)Immunohistochemical analysis of paraffin-embedded Mouse backskin labeling Dnmt3L with ab93613 at 1 ug/ml
-
![Immunocytochemistry/ Immunofluorescence - Anti-Dnmt3L antibody [N117/9] (ab93613)](https://www.abcam.com/ps/products/93/ab93613/Images/ab93613-260418-anti-dnmt3l-antibody-n117-9-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Dnmt3L antibody [N117/9] (ab93613)Immunofluorescent analysis on Human HaCaT cells labeling Dnmt3L with ab93613 at 1/1000 dilution.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab93613 has not yet been referenced specifically in any publications.
