Anti-Dnmt3L antibody [N117/9] (ab93613)
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- References
- Protocols
Overview
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Product name
Anti-Dnmt3L antibody [N117/9]
See all Dnmt3L primary antibodies -
Description
Mouse monoclonal [N117/9] to Dnmt3L -
Host species
Mouse -
Specificity
Detects Dnmt3L, it does not react with DNMT3a or DNMT3B. -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, IHC-Frmore details -
Species reactivity
Reacts with: Human -
Immunogen
Fusion protein, corresponding to amino acids 1-387 of Human Dnmt3L (accession number BAA95556)
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General notes
The clone number has been updated from S117-9 to N117/9, both clone numbers name the same antibody clone.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 50% Glycerol, PBS, pH 7.4 -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
N117/9 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab93613 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | ||
| IHC-P | ||
| ICC/IF | ||
| IHC-Fr |
IHC-P: Use at a concentration of 0.1 - 1 µg/ml.
IHC-Fr: Use at a concentration of 0.1 - 1 µg/ml.
WB: Use at a concentration of 1 - 10 µg/ml. Predicted molecular weight: 44 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Catalytically inactive regulatory factor of DNA methyltransferases. It is essential for the function of DNMT3A and DNMT3B. Activates DNMT3A and DNMT3B by binding to their catalytic domain. Accelerates the binding of DNA and AdoMet to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases. Recognizes unmethylated histone H3 lysine 4 (H3K4) and induces de novo DNA methylation by recruitment or activation of DNMT3. -
Tissue specificity
Expressed at low levels in several tissues including testis, ovary, and thymus. -
Sequence similarities
Belongs to the C5-methyltransferase family.
Contains 1 ADD-type zinc finger. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 29947 Human
- Omim: 606588 Human
- SwissProt: Q9UJW3 Human
- Unigene: 592165 Human
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Alternative names
- Cytosine 5 methyltransferase 3 like protein antibody
- DNA (cytosine 5 ) methyltransferase 3 like antibody
- DNA (cytosine-5)-methyltransferase 3-like antibody
see all
Images
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![Western blot - Anti-Dnmt3L antibody [N117/9] (ab93613)](https://www.abcam.com/ps/products/93/ab93613/Images/ab93613-199671-anti-dnmt3l-antibody-n117-9-western-blot.jpg)
Western blot - Anti-Dnmt3L antibody [N117/9] (ab93613)Western blot detection of DNMT3L in a Human tissue mix using ab93613 at 1:1000 dilution. -
![Immunohistochemistry (Frozen sections) - Anti-Dnmt3L antibody [N117/9] (ab93613)](https://www.abcam.com/ps/products/93/ab93613/Images/ab93613-260419-anti-dnmt3l-antibody-n117-9-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-Dnmt3L antibody [N117/9] (ab93613)Immunohistochemical analysis of paraffin-embedded Mouse backskin labeling Dnmt3L with ab93613 at 1 ug/ml
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![Immunocytochemistry/ Immunofluorescence - Anti-Dnmt3L antibody [N117/9] (ab93613)](https://www.abcam.com/ps/products/93/ab93613/Images/ab93613-260418-anti-dnmt3l-antibody-n117-9-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Dnmt3L antibody [N117/9] (ab93613)Immunofluorescent analysis on Human HaCaT cells labeling Dnmt3L with ab93613 at 1/1000 dilution.
Datasheets and documents
References
ab93613 has not yet been referenced specifically in any publications.
