Product nameDNQX, AMPA / kainate antagonist
DescriptionAMPA / kainate antagonist
Biological descriptionAMPA / kainate antagonist
Storage instructionsStore at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months.
Solubility overviewSoluble in DMSO to 100 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Our Abpromise guarantee covers the use of ab120018 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab96379 staining MEK1 (phospho S298) in SK-N-SH cells treated with DNQX (ab120018), by ICC/IF. Decrease in MEK1 (phospho S298) expression correlates with increased concentration of DNQX, as described in literature.
The cells were incubated at 37°C for 1h in media containing different concentrations of ab120018 (DNQX) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96379 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab120018 has been referenced in 40 publications.
- Gu F et al. Partial TrkB receptor activation suppresses cortical epileptogenesis through actions on parvalbumin interneurons. Neurobiol Dis 113:45-58 (2018). PubMed: 29408225
- Chittajallu R et al. Afferent specific role of NMDA receptors for the circuit integration of hippocampal neurogliaform cells. Nat Commun 8:152 (2017). PubMed: 28751664
- Cellot G et al. Premature changes in neuronal excitability account for hippocampal network impairment and autistic-like behavior in neonatal BTBR T+tf/J mice. Sci Rep 6:31696 (2016). PubMed: 27526668
- Mamaligas AA & Ford CP Spontaneous Synaptic Activation of Muscarinic Receptors by Striatal Cholinergic Neuron Firing. Neuron 91:574-86 (2016). PubMed: 27373830
- Sametsky EA et al. Enhanced GABAA-Mediated Tonic Inhibition in Auditory Thalamus of Rats with Behavioral Evidence of Tinnitus. J Neurosci 35:9369-80 (2015). PubMed: 26109660