Key features and details
- Rabbit polyclonal to dNT-2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-dNT-2 antibody
DescriptionRabbit polyclonal to dNT-2
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment (His-T7-tag) corresponding to Human dNT-2 aa 38-228. Expressed in E.coli.
VLVDMDGVLADFEGGFLRKFRARFPDQPFIALEDRRGFWVSEQYGRLRPG LSEKAISIWESKNFFFELEPLPGAVEAVKEMASLQNTDVFICTSPIKMFK YCPYEKYAWVEKYFGPDFLEQIVLTRDKTVVSADLLIDDRPDITGAEPTP SWEHVLFTACHNQHLQLQPPRRRLHSWADDWKAILDSKRPC
Database link: Q9NPB1
- IHC-P: Human brain tissue. WB: Mouse cerebrum lysate; Recombinant human dNT-2 protein.
Previously labelled as NT5M.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab231053 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab231053 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 26 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionDephosphorylates specifically the 5' and 2'(3')-phosphates of uracil and thymine deoxyribonucleotides, and so protects mitochondrial DNA replication from excess dTTP. Has only marginal activity towards dIMP and dGMP.
Tissue specificityHighly expressed in heart, brain and skeletal muscle. Detected at very low levels in kidney and pancreas.
Sequence similaritiesBelongs to the 5'(3')-deoxyribonucleotidase family.
- Information by UniProt
- 3''-nucleotidase antibody
- 5'' antibody
- 5''(3'')-deoxyribonucleotidase antibody
Formalin-fixed, paraffin-embedded human brain tissue stained for dNT-2 using ab231053 at 20 µg/ml in immunohistochemical analysis, followed by DAB staining.
Anti-dNT-2 antibody (ab231053) at 2 µg/ml + Mouse cerebrum lysate
Predicted band size: 26 kDa
Anti-dNT-2 antibody (ab231053) at 2 µg/ml + Recombinant human dNT-2 protein
Predicted band size: 26 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231053 has not yet been referenced specifically in any publications.