Anti-DOC2B antibody (ab234643)
Key features and details
- Rabbit polyclonal to DOC2B
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DOC2B antibody -
Description
Rabbit polyclonal to DOC2B -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment corresponding to Human DOC2B aa 1-200.
Database link: Q14184 -
Positive control
- IHC-P: Human placenta tissue. ICC/IF: HepG2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab234643 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/20 - 1/200.
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|
ICC/IF |
1/50 - 1/200.
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Notes |
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IHC-P
1/20 - 1/200. |
ICC/IF
1/50 - 1/200. |
Target
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Function
Calcium sensor which positively regulates SNARE-dependent fusion of vesicles with membranes. Binds phospholipids in a calcium-dependent manner and may act at the priming stage of fusion by modifying membrane curvature to stimulate fusion. Involved in calcium-triggered exocytosis in chromaffin cells and calcium-dependent spontaneous release of neurotransmitter in absence of action potentials in neuronal cells. Involved both in glucose-stimulated insulin secretion in pancreatic cells and insulin-dependent GLUT4 transport to the plasma membrane in adipocytes. -
Tissue specificity
Widely expressed with highest levels in brain and kidney. Expressed in pancreatic islet cells (at protein level). -
Sequence similarities
Contains 2 C2 domains. -
Domain
C2 domain 1 is involved in binding calcium and phospholipids. C2 domain 2 may also play a role in the calcium-dependent targeting to membranes. -
Cellular localization
Cytoplasm. Cytoplasmic granule. Cell membrane. Translocates to the plasma membrane in a calcium-dependent manner. - Information by UniProt
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Database links
- Entrez Gene: 8447 Human
- Entrez Gene: 13447 Mouse
- Entrez Gene: 81820 Rat
- Omim: 604568 Human
- SwissProt: Q14184 Human
- SwissProt: P70169 Mouse
- SwissProt: P70610 Rat
- Unigene: 551705 Human
see all -
Alternative names
- Doc2 beta antibody
- Doc2-beta antibody
- Doc2b antibody
see all
Images
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Paraffin-embedded human placenta tissue stained for DOC2B using ab234643 at 1/100 dilution in immunohistochemical analysis.
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HepG2 cells (Human liver hepatocellular carcinoma cell line) cells stained for DOC2B (green) using ab234643 at 1/100 dilution in ICC/IF, followed by Alexa-Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab234643 has not yet been referenced specifically in any publications.